Derivation and characterization of rabbit embryonic stem cells: A review

Elen Gócza, Z. Bösze

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

In the first part of this chapter the different types of pluripotent stem cells are described in general: embryonal carcinoma cells, mouse and human embryonic stem cells, germ cells, epiblast stem cells and induced pluripotent cells. The methods used for isolation of rabbit embryonic stem like cells and rabbit primordial germ cells are detailed in the second part, including the species specific factors playing role in the maintenance of pluripotency. Detection of pluripotency markers both at mRNA and protein levels is an important tool in embryonic stem cell characterization. In vitro differentiation, teratoma formation and chimera forming ability are all inevitable tools to characterize embryonic stem cells. Finally the future potential of a truly validated widely available rabbit embryonic stem cell line is highlighted.

Original languageEnglish
Title of host publicationRabbit Biotechnology: Rabbit Genomics, Transgenesis, Cloning and Models
PublisherSpringer Netherlands
Pages77-104
Number of pages28
ISBN (Print)9789048122264
DOIs
Publication statusPublished - 2009

Fingerprint

Embryonic Stem Cells
Rabbits
Germ Cells
Embryonal Carcinoma Stem Cells
Germ Layers
Induced Pluripotent Stem Cells
Pluripotent Stem Cells
Teratoma
Maintenance
Cell Line
Messenger RNA
Proteins

Keywords

  • chimera
  • Embryonic stem cells
  • in vitro differentiation
  • pluripotency

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

Cite this

Gócza, E., & Bösze, Z. (2009). Derivation and characterization of rabbit embryonic stem cells: A review. In Rabbit Biotechnology: Rabbit Genomics, Transgenesis, Cloning and Models (pp. 77-104). Springer Netherlands. https://doi.org/10.1007/978-90-481-2227-1_9

Derivation and characterization of rabbit embryonic stem cells : A review. / Gócza, Elen; Bösze, Z.

Rabbit Biotechnology: Rabbit Genomics, Transgenesis, Cloning and Models. Springer Netherlands, 2009. p. 77-104.

Research output: Chapter in Book/Report/Conference proceedingChapter

Gócza, E & Bösze, Z 2009, Derivation and characterization of rabbit embryonic stem cells: A review. in Rabbit Biotechnology: Rabbit Genomics, Transgenesis, Cloning and Models. Springer Netherlands, pp. 77-104. https://doi.org/10.1007/978-90-481-2227-1_9
Gócza E, Bösze Z. Derivation and characterization of rabbit embryonic stem cells: A review. In Rabbit Biotechnology: Rabbit Genomics, Transgenesis, Cloning and Models. Springer Netherlands. 2009. p. 77-104 https://doi.org/10.1007/978-90-481-2227-1_9
Gócza, Elen ; Bösze, Z. / Derivation and characterization of rabbit embryonic stem cells : A review. Rabbit Biotechnology: Rabbit Genomics, Transgenesis, Cloning and Models. Springer Netherlands, 2009. pp. 77-104
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