Derailed B cell homeostasis in patients with mixed connective tissue disease

A. Hajas, S. Baráth, P. Szodoray, B. Nakken, P. Gogolák, Z. Szekanecz, E. Zold, M. Zeher, G. Szegedi, E. Bodolay

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Abstract

Mixed connective tissue disease (MCTD) is a systemic autoimmune disorder, characterized by the presence of antibodies to U1-RNP protein. We aimed to determine phenotypic abnormalities of peripheral B cell subsets in MCTD. Blood samples were obtained from 46 MCTD patients, and 20 controls. Using anti-CD19, anti-CD27, anti-IgD and anti-CD38 monoclonal antibodies, the following B cell subsets were identified by flow cytometry: (1) transitional B cells (CD19+CD27-IgD+CD38high); (2) naive B cells (CD19+CD27-IgD+CD38low); (3) non-switched memory B cells (CD19+CD27+IgD+); (4) switched memory B cells (CD19+CD27+IgD-); (5) double negative (DN) memory B cells (CD19+CD27-IgD-) and (6) plasma cells (CD19+CD27highIgD-). The proportion of transitional B cells, naive B cells and DN B lymphocytes was higher in MCTD than in controls. The DN B cells were positive for CD95 surface marker. This memory B cells population showed a close correlation with disease activity. The number of plasma cells was also increased, and there was an association between the number of plasma cells and the anti-U1RNP levels. Cyclophosphamide, methotrexate, and corticosteroid treatment decreased the number of DN and CD27high B cells. In conclusion, several abnormalities were found in the peripheral B-cell subsets in MCTD, which reinforces the role of derailed humoral autoimmune processes in the pathogenesis.

Original languageEnglish
Pages (from-to)833-841
Number of pages9
JournalHuman Immunology
Volume74
Issue number7
DOIs
Publication statusPublished - Jul 2013

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Mixed Connective Tissue Disease
Homeostasis
B-Lymphocytes
Immunoglobulin D
B-Lymphocyte Subsets
Plasma Cells
B-Lymphoid Precursor Cells
Methotrexate
Cyclophosphamide
Flow Cytometry
Adrenal Cortex Hormones
Monoclonal Antibodies

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Derailed B cell homeostasis in patients with mixed connective tissue disease. / Hajas, A.; Baráth, S.; Szodoray, P.; Nakken, B.; Gogolák, P.; Szekanecz, Z.; Zold, E.; Zeher, M.; Szegedi, G.; Bodolay, E.

In: Human Immunology, Vol. 74, No. 7, 07.2013, p. 833-841.

Research output: Contribution to journalArticle

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