Depolarization promotes caffeine induced [3H]-noradrenaline release in calcium-free solution from peripheral sympathetic nerves

P. T. Tóth, T. L. Török, K. Magyar

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The transmitter releasing action of caffeine was studied in the absence of extracellular Ca2+ from the peripheral sympathetic nerves of the rabbit main pulmonary artery. Caffeine (10 mM) increased the release of [3H]-noradrenaline moderately, but not significantly in Ca2+-free (+1 mM EGTA) Krebs solution. When peripheral nerve endings/varicoslties were depolarized by elevating extracellular K+ to 47.2 mM and 70.8 mM in Ca2+-free solution, the transmitter releasing effect of 10 mM caffeine became significant. Ca2+ removal itself transiently increased the [3H]-noradrenaline outflow. In the individual experiments the amount of the caffeine evoked transmitter release at 47.2 mM and 70.8 mM K+-depolarization was inversely correlated to the release evoked by Ca2+-removal. Our results suggest that caffeine-sensitive calcium stores are present in peripheral nerve terminals of rabbit pulmonary artery, and part of the caffeine sensitive calcium stores may discharge during Ca2+-removal from the extracellular solution.

Original languageEnglish
Pages (from-to)557-563
Number of pages7
JournalCell Calcium
Volume11
Issue number9
DOIs
Publication statusPublished - 1990

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Caffeine
Peripheral Nerves
Norepinephrine
Calcium
Pulmonary Artery
Rabbits
Nerve Endings
Egtazic Acid

ASJC Scopus subject areas

  • Cell Biology
  • Endocrinology

Cite this

Depolarization promotes caffeine induced [3H]-noradrenaline release in calcium-free solution from peripheral sympathetic nerves. / Tóth, P. T.; Török, T. L.; Magyar, K.

In: Cell Calcium, Vol. 11, No. 9, 1990, p. 557-563.

Research output: Contribution to journalArticle

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