Depolarization of the membrane potential by β-lactams as a signal to induce autolysis

András Penyige, J. Matkó, Eleonóra Deák, Andrea Bodnár, György Barabás

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The effect of β-lactam antibiotics that are known to inhibit cell wall biosynthesis and induce cell wall autolysis on the electrophysiological state of the plasma membrane in Streptomyces griseus was studied. Addition of various β-lactam antibiotics induced a dose- and growth-stage-dependent depolarization of the membrane potential of Streptomyces griseus. The hydrolyzed biologically inactive derivative penicilloic acid had no depolarizing effect on the membrane potential. The ionophore gramicidin D, while depolarizing the membrane potential, also induced a dose-dependent increase in cell wall lysis. These observations suggest that alteration of the transmembrane potential could be an important signal in triggering cell wall autolysis of S. griseus.

Original languageEnglish
Pages (from-to)1169-1175
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume290
Issue number4
DOIs
Publication statusPublished - 2002

Fingerprint

Autolysis
Lactams
Depolarization
Streptomyces griseus
Membrane Potentials
Cell Wall
Cells
Membranes
Anti-Bacterial Agents
Gramicidin
Biosynthesis
Ionophores
Cell membranes
Cell Membrane
Derivatives
Growth

Keywords

  • DiOC(3)
  • Effect of penicillin G, cephotaxime, gramicidin D
  • Fluorescence assay of bacterial membrane potential
  • Induction of cell wall autolysis
  • Kanamycin
  • Mechanism of action of β-lactam antibiotics
  • Protoplasts
  • Streptomyces griseus

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Depolarization of the membrane potential by β-lactams as a signal to induce autolysis. / Penyige, András; Matkó, J.; Deák, Eleonóra; Bodnár, Andrea; Barabás, György.

In: Biochemical and Biophysical Research Communications, Vol. 290, No. 4, 2002, p. 1169-1175.

Research output: Contribution to journalArticle

Penyige, András ; Matkó, J. ; Deák, Eleonóra ; Bodnár, Andrea ; Barabás, György. / Depolarization of the membrane potential by β-lactams as a signal to induce autolysis. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 290, No. 4. pp. 1169-1175.
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