Depolarization of in situ mitochondria due to hydrogen peroxide-induced oxidative stress in nerve terminals: Inhibition of α-ketoglutarate dehydrogenase

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Abstract

Mitochondrial membrane potential (ΔΨ(m)) was determined in intact isolated nerve terminals using the membrane potential-sensitive probe JC-1. Oxidative stress induced by H2O2 (0.1-1 mM) caused only a minor decrease in ΔΨ(m). When complex I of the respiratory chain was inhibited by rotenone (2 μM), ΔΨ(m) was unaltered, but on subsequent addition of H2O2, ΔΨ(m) started to decrease and collapsed during incubation with 0.5 mM H2O2 for 12 min. The ATP level and [ATP]/[ADP] ratio were greatly reduced in the simultaneous presence of rotenone and H2O2. H2O2 also induced a marked reduction in ΔΨ(m) when added after oligomycin (10 μM), an inhibitor of F0F1-ATPase. H2O2 (0.1 or 0.5 mM) inhibited α-ketoglutarate dehydrogenase and decreased the steady-state NAD(P)H level in nerve terminals. It is concluded that there are at least two factors that determine ΔΨ(m) in the presence of H2O2: (a) The NADH level reduced owing to inhibition of α-ketoglutarate dehydrogenase is insufficient to ensure an optimal rate of respiration, which is reflected in a fall of ΔΨ(m) when the F0F1-ATPase is not functional. (b) The greatly reduced ATP level in the presence of rotenone and H2O2 prevents maintenance of ΔΨ(m) by F0F1- ATPase. The results indicate that to maintain ΔΨ(m) in the nerve terminal during H2O2-induced oxidative stress, both complex I and F0F1-ATPase must be functional. Collapse of ΔΨ(m) could be a critical event in neuronal injury in ischemia or Parkinson's disease when H2O2 is generated in excess and complex I of the respiratory chain is simultaneously impaired.

Original languageEnglish
Pages (from-to)220-228
Number of pages9
JournalJournal of Neurochemistry
Volume73
Issue number1
DOIs
Publication statusPublished - 1999

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Mitochondria
Oxidative stress
Proton-Translocating ATPases
Depolarization
Rotenone
Hydrogen Peroxide
Oxidoreductases
Oxidative Stress
Adenosine Triphosphate
Electron Transport
NAD
Membranes
Oligomycins
Mitochondrial Membrane Potential
Respiratory Rate
Membrane Potentials
Adenosine Diphosphate
Parkinson Disease
Ischemia
Maintenance

Keywords

  • α-Ketoglutarate dehydrogenase
  • Hydrogen peroxide
  • Mitochondrial ATPase
  • Mitochondrial membrane potential
  • Oxidative stress
  • Parkinson's disease

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

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title = "Depolarization of in situ mitochondria due to hydrogen peroxide-induced oxidative stress in nerve terminals: Inhibition of α-ketoglutarate dehydrogenase",
abstract = "Mitochondrial membrane potential (ΔΨ(m)) was determined in intact isolated nerve terminals using the membrane potential-sensitive probe JC-1. Oxidative stress induced by H2O2 (0.1-1 mM) caused only a minor decrease in ΔΨ(m). When complex I of the respiratory chain was inhibited by rotenone (2 μM), ΔΨ(m) was unaltered, but on subsequent addition of H2O2, ΔΨ(m) started to decrease and collapsed during incubation with 0.5 mM H2O2 for 12 min. The ATP level and [ATP]/[ADP] ratio were greatly reduced in the simultaneous presence of rotenone and H2O2. H2O2 also induced a marked reduction in ΔΨ(m) when added after oligomycin (10 μM), an inhibitor of F0F1-ATPase. H2O2 (0.1 or 0.5 mM) inhibited α-ketoglutarate dehydrogenase and decreased the steady-state NAD(P)H level in nerve terminals. It is concluded that there are at least two factors that determine ΔΨ(m) in the presence of H2O2: (a) The NADH level reduced owing to inhibition of α-ketoglutarate dehydrogenase is insufficient to ensure an optimal rate of respiration, which is reflected in a fall of ΔΨ(m) when the F0F1-ATPase is not functional. (b) The greatly reduced ATP level in the presence of rotenone and H2O2 prevents maintenance of ΔΨ(m) by F0F1- ATPase. The results indicate that to maintain ΔΨ(m) in the nerve terminal during H2O2-induced oxidative stress, both complex I and F0F1-ATPase must be functional. Collapse of ΔΨ(m) could be a critical event in neuronal injury in ischemia or Parkinson's disease when H2O2 is generated in excess and complex I of the respiratory chain is simultaneously impaired.",
keywords = "α-Ketoglutarate dehydrogenase, Hydrogen peroxide, Mitochondrial ATPase, Mitochondrial membrane potential, Oxidative stress, Parkinson's disease",
author = "C. Chinopoulos and L. Tretter and V. {\'A}d{\'a}m-Vizi",
year = "1999",
doi = "10.1046/j.1471-4159.1999.0730220.x",
language = "English",
volume = "73",
pages = "220--228",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
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TY - JOUR

T1 - Depolarization of in situ mitochondria due to hydrogen peroxide-induced oxidative stress in nerve terminals

T2 - Inhibition of α-ketoglutarate dehydrogenase

AU - Chinopoulos, C.

AU - Tretter, L.

AU - Ádám-Vizi, V.

PY - 1999

Y1 - 1999

N2 - Mitochondrial membrane potential (ΔΨ(m)) was determined in intact isolated nerve terminals using the membrane potential-sensitive probe JC-1. Oxidative stress induced by H2O2 (0.1-1 mM) caused only a minor decrease in ΔΨ(m). When complex I of the respiratory chain was inhibited by rotenone (2 μM), ΔΨ(m) was unaltered, but on subsequent addition of H2O2, ΔΨ(m) started to decrease and collapsed during incubation with 0.5 mM H2O2 for 12 min. The ATP level and [ATP]/[ADP] ratio were greatly reduced in the simultaneous presence of rotenone and H2O2. H2O2 also induced a marked reduction in ΔΨ(m) when added after oligomycin (10 μM), an inhibitor of F0F1-ATPase. H2O2 (0.1 or 0.5 mM) inhibited α-ketoglutarate dehydrogenase and decreased the steady-state NAD(P)H level in nerve terminals. It is concluded that there are at least two factors that determine ΔΨ(m) in the presence of H2O2: (a) The NADH level reduced owing to inhibition of α-ketoglutarate dehydrogenase is insufficient to ensure an optimal rate of respiration, which is reflected in a fall of ΔΨ(m) when the F0F1-ATPase is not functional. (b) The greatly reduced ATP level in the presence of rotenone and H2O2 prevents maintenance of ΔΨ(m) by F0F1- ATPase. The results indicate that to maintain ΔΨ(m) in the nerve terminal during H2O2-induced oxidative stress, both complex I and F0F1-ATPase must be functional. Collapse of ΔΨ(m) could be a critical event in neuronal injury in ischemia or Parkinson's disease when H2O2 is generated in excess and complex I of the respiratory chain is simultaneously impaired.

AB - Mitochondrial membrane potential (ΔΨ(m)) was determined in intact isolated nerve terminals using the membrane potential-sensitive probe JC-1. Oxidative stress induced by H2O2 (0.1-1 mM) caused only a minor decrease in ΔΨ(m). When complex I of the respiratory chain was inhibited by rotenone (2 μM), ΔΨ(m) was unaltered, but on subsequent addition of H2O2, ΔΨ(m) started to decrease and collapsed during incubation with 0.5 mM H2O2 for 12 min. The ATP level and [ATP]/[ADP] ratio were greatly reduced in the simultaneous presence of rotenone and H2O2. H2O2 also induced a marked reduction in ΔΨ(m) when added after oligomycin (10 μM), an inhibitor of F0F1-ATPase. H2O2 (0.1 or 0.5 mM) inhibited α-ketoglutarate dehydrogenase and decreased the steady-state NAD(P)H level in nerve terminals. It is concluded that there are at least two factors that determine ΔΨ(m) in the presence of H2O2: (a) The NADH level reduced owing to inhibition of α-ketoglutarate dehydrogenase is insufficient to ensure an optimal rate of respiration, which is reflected in a fall of ΔΨ(m) when the F0F1-ATPase is not functional. (b) The greatly reduced ATP level in the presence of rotenone and H2O2 prevents maintenance of ΔΨ(m) by F0F1- ATPase. The results indicate that to maintain ΔΨ(m) in the nerve terminal during H2O2-induced oxidative stress, both complex I and F0F1-ATPase must be functional. Collapse of ΔΨ(m) could be a critical event in neuronal injury in ischemia or Parkinson's disease when H2O2 is generated in excess and complex I of the respiratory chain is simultaneously impaired.

KW - α-Ketoglutarate dehydrogenase

KW - Hydrogen peroxide

KW - Mitochondrial ATPase

KW - Mitochondrial membrane potential

KW - Oxidative stress

KW - Parkinson's disease

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