Dependence of wheat callus growth, differentiation and mineral content on carbohydrate supply

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Abstract

The effects of different carbon sources on the growth, differentiation and mineral content of wheat callus were investigated. Callus originating from immature embryos showed optimal growth produced the highest ratio of shoots when it was cultured on the medium containing 0.058 M sucrose. Higher carbohydrate concentrations reduced both shoot formation and growth. On the other hand, when sucrose concentration was less than 0.029 M neither differentiation nor greening was observed. Mannitol had a stimulating effect on shoot formation when the medium containing 0.029 M sucrose was supplemented by mannitol to get the final concentration of 0.058 M. The respiration rate increased along with increasing concentration of sucrose and glucose, and reached a maximum in the case of sucrose at the concentration of 0.263 M. On the addition of different concentrations of mannitol to a 0.058 M sucrose medium the respiration remained essentially unchanged. The mineral content of the tissue cultures also depended on the carbohydrate concentration. The water content decreased with increasing carbohydrate concentrations and among carbohydrates examined, sucrose was the most effective. The nitrogen and potassium contents of the calli reached their maximum values at 0.117 M-0.175 M carbohydrate content. The highest phosphorus contents were detected at 0.350 M-0.468 M carbohydrates. Phosphorus proved to be the most sensitive to osmotic changes.

Original languageEnglish
Pages (from-to)65-70
Number of pages6
JournalPlant Science
Volume45
Issue number1
DOIs
Publication statusPublished - 1986

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Keywords

  • carbohydrates
  • differentiation
  • osmotic adjustment
  • water and ion content
  • wheat tissue culture

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

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