Dependence of chlorophyll fluorescence quenching on the lipid-to-protein ratio in reconstituted light-harvesting complex II membranes containing lipid labels

Parveen Akhtar, Fanni Görföl, G. Garab, P. Lambrev

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The quenching of chlorophyll-a fluorescence was investigated in plant light-harvesting complex II (LHCII) embedded in reconstituted membranes containing thylakoid lipids and a lipid label. The proteoliposomes were further separated by density and the protein and lipid contents of the fractions were quantified spectrophotometrically, allowing tighter control over the L/P ratios in a wide range of values. Using time-resolved fluorescence, we found a strong correlation between the fluorescence quenching and the L/P ratio, in line with other studies reporting progressive quenching at low L/P ratios, presumably triggered by self-clustering in the membrane. The average fluorescence lifetimes decreased to 0.3 ns at L/P ratios below 50:1; these values are comparable to the quenching observed in plants under excess-light conditions and are accompanied by a similar far-red fluorescence signature. It is hypothesized that plants can exploit the intrinsic quenching propensity of LHCII-only membrane domains to safely store extra antenna units.

Original languageEnglish
Pages (from-to)242-248
Number of pages7
JournalChemical Physics
Volume522
DOIs
Publication statusPublished - Jun 1 2019

Fingerprint

chlorophylls
Chlorophyll
lipids
Labels
Quenching
Fluorescence
quenching
membranes
proteins
Membranes
Lipids
fluorescence
Proteins
antennas
signatures
Antennas
life (durability)

Keywords

  • Circular dichroism
  • Non-photochemical quenching
  • Protein-lipid interactions
  • Proteoliposomes
  • Thylakoid lipids
  • Time-resolved fluorescence

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

Cite this

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title = "Dependence of chlorophyll fluorescence quenching on the lipid-to-protein ratio in reconstituted light-harvesting complex II membranes containing lipid labels",
abstract = "The quenching of chlorophyll-a fluorescence was investigated in plant light-harvesting complex II (LHCII) embedded in reconstituted membranes containing thylakoid lipids and a lipid label. The proteoliposomes were further separated by density and the protein and lipid contents of the fractions were quantified spectrophotometrically, allowing tighter control over the L/P ratios in a wide range of values. Using time-resolved fluorescence, we found a strong correlation between the fluorescence quenching and the L/P ratio, in line with other studies reporting progressive quenching at low L/P ratios, presumably triggered by self-clustering in the membrane. The average fluorescence lifetimes decreased to 0.3 ns at L/P ratios below 50:1; these values are comparable to the quenching observed in plants under excess-light conditions and are accompanied by a similar far-red fluorescence signature. It is hypothesized that plants can exploit the intrinsic quenching propensity of LHCII-only membrane domains to safely store extra antenna units.",
keywords = "Circular dichroism, Non-photochemical quenching, Protein-lipid interactions, Proteoliposomes, Thylakoid lipids, Time-resolved fluorescence",
author = "Parveen Akhtar and Fanni G{\"o}rf{\"o}l and G. Garab and P. Lambrev",
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T1 - Dependence of chlorophyll fluorescence quenching on the lipid-to-protein ratio in reconstituted light-harvesting complex II membranes containing lipid labels

AU - Akhtar, Parveen

AU - Görföl, Fanni

AU - Garab, G.

AU - Lambrev, P.

PY - 2019/6/1

Y1 - 2019/6/1

N2 - The quenching of chlorophyll-a fluorescence was investigated in plant light-harvesting complex II (LHCII) embedded in reconstituted membranes containing thylakoid lipids and a lipid label. The proteoliposomes were further separated by density and the protein and lipid contents of the fractions were quantified spectrophotometrically, allowing tighter control over the L/P ratios in a wide range of values. Using time-resolved fluorescence, we found a strong correlation between the fluorescence quenching and the L/P ratio, in line with other studies reporting progressive quenching at low L/P ratios, presumably triggered by self-clustering in the membrane. The average fluorescence lifetimes decreased to 0.3 ns at L/P ratios below 50:1; these values are comparable to the quenching observed in plants under excess-light conditions and are accompanied by a similar far-red fluorescence signature. It is hypothesized that plants can exploit the intrinsic quenching propensity of LHCII-only membrane domains to safely store extra antenna units.

AB - The quenching of chlorophyll-a fluorescence was investigated in plant light-harvesting complex II (LHCII) embedded in reconstituted membranes containing thylakoid lipids and a lipid label. The proteoliposomes were further separated by density and the protein and lipid contents of the fractions were quantified spectrophotometrically, allowing tighter control over the L/P ratios in a wide range of values. Using time-resolved fluorescence, we found a strong correlation between the fluorescence quenching and the L/P ratio, in line with other studies reporting progressive quenching at low L/P ratios, presumably triggered by self-clustering in the membrane. The average fluorescence lifetimes decreased to 0.3 ns at L/P ratios below 50:1; these values are comparable to the quenching observed in plants under excess-light conditions and are accompanied by a similar far-red fluorescence signature. It is hypothesized that plants can exploit the intrinsic quenching propensity of LHCII-only membrane domains to safely store extra antenna units.

KW - Circular dichroism

KW - Non-photochemical quenching

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KW - Proteoliposomes

KW - Thylakoid lipids

KW - Time-resolved fluorescence

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