Deoxycytidine kinase can be also potentiated by the G-protein activator NaF in cells

M. Staub, Z. Csapó, T. Spasokukotskaja, M. Sasvári

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Recently, it has been shown, that 2-Chloro-deoyadenoise (1), a series of analogies, an other DNA synthesis inhibitors, increased the deoxycytidine kinase (dCK) enzyme activity in different cells, without influencing thymidine kinase isoenzymes (TK1, TK2), dCMP-deaminase and thymidylate synthase (TS) activities (2,3). The dCK activity was 2-4 times higher in analogue treated cells, than in controls, which can not be explained by metabolic pool imbalance induced by the drugs. New mRNA and protein synthesis of dCK could not be detected, thus post-translational modification has been suggested for potenciatiation the activity of the dCK (1). Because secondary modifications of enzymes usually involve the signalling processes in cells, the universal G-protein activator fluorine ions were tested. dCK activity of human lymphnode lymphocytes were increased 2-times, if cells were incubated in the presence of NaF for 1-2 hrs in cultures, while TK activity was not changed. The formation of dUTP from dCyd, was also enhanced by NaF, in paralel of dCK potentiation.

Original languageEnglish
Pages (from-to)425-428
Number of pages4
JournalAdvances in Experimental Medicine and Biology
Volume431
Publication statusPublished - 1998

Fingerprint

Deoxycytidine Kinase
GTP-Binding Proteins
Nucleic Acid Synthesis Inhibitors
Thymidylate Synthase
Lymphocytes
Thymidine Kinase
Fluorine
Enzyme activity
Enzymes
Post Translational Protein Processing
Human Activities
Isoenzymes
Cells
Ions
Messenger RNA
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Deoxycytidine kinase can be also potentiated by the G-protein activator NaF in cells. / Staub, M.; Csapó, Z.; Spasokukotskaja, T.; Sasvári, M.

In: Advances in Experimental Medicine and Biology, Vol. 431, 1998, p. 425-428.

Research output: Contribution to journalArticle

@article{d1a86d57514c48a8b16de1e0d0d03c23,
title = "Deoxycytidine kinase can be also potentiated by the G-protein activator NaF in cells",
abstract = "Recently, it has been shown, that 2-Chloro-deoyadenoise (1), a series of analogies, an other DNA synthesis inhibitors, increased the deoxycytidine kinase (dCK) enzyme activity in different cells, without influencing thymidine kinase isoenzymes (TK1, TK2), dCMP-deaminase and thymidylate synthase (TS) activities (2,3). The dCK activity was 2-4 times higher in analogue treated cells, than in controls, which can not be explained by metabolic pool imbalance induced by the drugs. New mRNA and protein synthesis of dCK could not be detected, thus post-translational modification has been suggested for potenciatiation the activity of the dCK (1). Because secondary modifications of enzymes usually involve the signalling processes in cells, the universal G-protein activator fluorine ions were tested. dCK activity of human lymphnode lymphocytes were increased 2-times, if cells were incubated in the presence of NaF for 1-2 hrs in cultures, while TK activity was not changed. The formation of dUTP from dCyd, was also enhanced by NaF, in paralel of dCK potentiation.",
author = "M. Staub and Z. Csap{\'o} and T. Spasokukotskaja and M. Sasv{\'a}ri",
year = "1998",
language = "English",
volume = "431",
pages = "425--428",
journal = "Advances in Experimental Medicine and Biology",
issn = "0065-2598",
publisher = "Springer New York",

}

TY - JOUR

T1 - Deoxycytidine kinase can be also potentiated by the G-protein activator NaF in cells

AU - Staub, M.

AU - Csapó, Z.

AU - Spasokukotskaja, T.

AU - Sasvári, M.

PY - 1998

Y1 - 1998

N2 - Recently, it has been shown, that 2-Chloro-deoyadenoise (1), a series of analogies, an other DNA synthesis inhibitors, increased the deoxycytidine kinase (dCK) enzyme activity in different cells, without influencing thymidine kinase isoenzymes (TK1, TK2), dCMP-deaminase and thymidylate synthase (TS) activities (2,3). The dCK activity was 2-4 times higher in analogue treated cells, than in controls, which can not be explained by metabolic pool imbalance induced by the drugs. New mRNA and protein synthesis of dCK could not be detected, thus post-translational modification has been suggested for potenciatiation the activity of the dCK (1). Because secondary modifications of enzymes usually involve the signalling processes in cells, the universal G-protein activator fluorine ions were tested. dCK activity of human lymphnode lymphocytes were increased 2-times, if cells were incubated in the presence of NaF for 1-2 hrs in cultures, while TK activity was not changed. The formation of dUTP from dCyd, was also enhanced by NaF, in paralel of dCK potentiation.

AB - Recently, it has been shown, that 2-Chloro-deoyadenoise (1), a series of analogies, an other DNA synthesis inhibitors, increased the deoxycytidine kinase (dCK) enzyme activity in different cells, without influencing thymidine kinase isoenzymes (TK1, TK2), dCMP-deaminase and thymidylate synthase (TS) activities (2,3). The dCK activity was 2-4 times higher in analogue treated cells, than in controls, which can not be explained by metabolic pool imbalance induced by the drugs. New mRNA and protein synthesis of dCK could not be detected, thus post-translational modification has been suggested for potenciatiation the activity of the dCK (1). Because secondary modifications of enzymes usually involve the signalling processes in cells, the universal G-protein activator fluorine ions were tested. dCK activity of human lymphnode lymphocytes were increased 2-times, if cells were incubated in the presence of NaF for 1-2 hrs in cultures, while TK activity was not changed. The formation of dUTP from dCyd, was also enhanced by NaF, in paralel of dCK potentiation.

UR - http://www.scopus.com/inward/record.url?scp=0031836312&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031836312&partnerID=8YFLogxK

M3 - Article

C2 - 9598103

AN - SCOPUS:0031836312

VL - 431

SP - 425

EP - 428

JO - Advances in Experimental Medicine and Biology

JF - Advances in Experimental Medicine and Biology

SN - 0065-2598

ER -