Deoxycytidine is salvaged not only into DNA but also into phospholipid precursors II. Ara-C does not inhibit the later process in lymphoid cells

Mária Sasvári-Székely, Tatjana Spasokukotskaja, Ágnes Soóki-Tóth, G. Pogany, L. Kopper, Mária Staub

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Abstract

dCTP formed from exogenous deoxycytidine via the salvage pathways was previously shown to serve deoxyliponucleotide synthesis in lymphocytes (Spasokukotskaja et al, Biochem. Biophys. Res. Commun. (1988) 155, 923-929) and now in lymphoma cells. After treatment with 1-β-D-arabino-furanosylcytosine (ara-C), much more araCTP as well as araCDP-choline was formed in lymphoma cells than in lymphocytes explaining the high sensitivity of lymphoma cells to this drug. Ara-C did not inhibit labeling of 5-3H-dCDP-choline from exogenous 5-3H-deoxycytidine while inhibiting DNA synthesis. Excess of exogenous ribocytidine diminished labeling of araCDP-choline, without any effect on dCDP-choline. These data suggest that araCDP-choline and dCDP- choline were synthesized from separate pools in these cells.

Original languageEnglish
Pages (from-to)1158-1167
Number of pages10
JournalBiochemical and biophysical research communications
Volume163
Issue number2
DOIs
Publication statusPublished - Sep 15 1989

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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