Deoxycytidine is salvaged not only into DNA but also into phospholipid precursors. III. dCDP-diacylglycerol formation in tonsillar lymphocytes

Tatjana Spasokukotskaja, J. Taljanidisz, M. Sasvári-Székely, M. Staub

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In addition to the water-soluble deoxyliponucleotides (Spasokukotskaja et al. (1988), Biochem. Biophys. Res. Commun. 155, 923), a lipid compound was shown to be labeled from external 3H-deoxycytidine (5-3H-CdR) in infant tonsillar lymphocytes. Chlorpromazine enhanced the labeling of this compound, identified by TLC as 3H-dCDP-diacylglycerol (3H-dCDP-DAG). The deoxynucleotide salvage pathway seems to be the main source for dCDP-DAG synthesis, as hydroxyurea increased its labeling from CdR. myo-Inositol induced the disappearance of 5-3H-dCDP-DAG, suggesting its utilization for phosphatidylinositol synthesis. 3H-Arahinosyl-Cytosine (araC) is also incorporated into the lipidic fraction at a rate comparable to its incorporation into DNA, supporting the effect of araC on membrane functions.

Original languageEnglish
Pages (from-to)680-687
Number of pages8
JournalBiochemical and biophysical research communications
Volume174
Issue number2
DOIs
Publication statusPublished - Jan 31 1991

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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