Dendrite-Specific Amplification of Weak Synaptic Input during Network Activity In Vivo

Leiron Ferrarese, Jean Sébastien Jouhanneau, Michiel W.H. Remme, Jens Kremkow, Gergely Katona, B. Rózsa, Susanne Schreiber, James F.A. Poulet

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Excitatory synaptic input reaches the soma of a cortical excitatory pyramidal neuron via anatomically segregated apical and basal dendrites. In vivo, dendritic inputs are integrated during depolarized network activity, but how network activity affects apical and basal inputs is not understood. Using subcellular two-photon stimulation of Channelrhodopsin2-expressing layer 2/3 pyramidal neurons in somatosensory cortex, nucleus-specific thalamic optogenetic stimulation, and paired recordings, we show that slow, depolarized network activity amplifies small-amplitude synaptic inputs targeted to basal dendrites but reduces the amplitude of all inputs from apical dendrites and the cell soma. Intracellular pharmacology and mathematical modeling suggests that the amplification of weak basal inputs is mediated by postsynaptic voltage-gated channels. Thus, network activity dynamically reconfigures the relative somatic contribution of apical and basal inputs and could act to enhance the detectability of weak synaptic inputs. Ferrarese et al. investigate the impact of network activity on synaptic integration in cortical L2/3 pyramidal neurons in vivo. They report a reduction of apical dendritic inputs but an amplification of small-amplitude basal inputs during depolarized phases of slow network activity. The amplification is dependent on postsynaptic voltage-gated channels.

Original languageEnglish
Pages (from-to)3455-3465.e5
JournalCell Reports
Volume24
Issue number13
DOIs
Publication statusPublished - Sep 25 2018

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Pyramidal Cells
Dendrites
Neurons
Amplification
Carisoprodol
Optogenetics
Thalamic Nuclei
Somatosensory Cortex
Electric potential
Photons
Pharmacology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Ferrarese, L., Jouhanneau, J. S., Remme, M. W. H., Kremkow, J., Katona, G., Rózsa, B., ... Poulet, J. F. A. (2018). Dendrite-Specific Amplification of Weak Synaptic Input during Network Activity In Vivo. Cell Reports, 24(13), 3455-3465.e5. https://doi.org/10.1016/j.celrep.2018.08.088

Dendrite-Specific Amplification of Weak Synaptic Input during Network Activity In Vivo. / Ferrarese, Leiron; Jouhanneau, Jean Sébastien; Remme, Michiel W.H.; Kremkow, Jens; Katona, Gergely; Rózsa, B.; Schreiber, Susanne; Poulet, James F.A.

In: Cell Reports, Vol. 24, No. 13, 25.09.2018, p. 3455-3465.e5.

Research output: Contribution to journalArticle

Ferrarese, L, Jouhanneau, JS, Remme, MWH, Kremkow, J, Katona, G, Rózsa, B, Schreiber, S & Poulet, JFA 2018, 'Dendrite-Specific Amplification of Weak Synaptic Input during Network Activity In Vivo', Cell Reports, vol. 24, no. 13, pp. 3455-3465.e5. https://doi.org/10.1016/j.celrep.2018.08.088
Ferrarese L, Jouhanneau JS, Remme MWH, Kremkow J, Katona G, Rózsa B et al. Dendrite-Specific Amplification of Weak Synaptic Input during Network Activity In Vivo. Cell Reports. 2018 Sep 25;24(13):3455-3465.e5. https://doi.org/10.1016/j.celrep.2018.08.088
Ferrarese, Leiron ; Jouhanneau, Jean Sébastien ; Remme, Michiel W.H. ; Kremkow, Jens ; Katona, Gergely ; Rózsa, B. ; Schreiber, Susanne ; Poulet, James F.A. / Dendrite-Specific Amplification of Weak Synaptic Input during Network Activity In Vivo. In: Cell Reports. 2018 ; Vol. 24, No. 13. pp. 3455-3465.e5.
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