Demonstration of super-resolution microscopy using a high numerical-aperture oil-immersion objective lens

Yoshinori Iketaki, Takeshi Watanabe, N. Bokor, Masaaki Fujii

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We demonstrated super-resolution microscopy using a high numerical aperture (NA) oil immersion lens in acute optical alignment condition. In super-resolution microscopy, the pump beam is overlapped together with a doughnut-shaped erase beam in the focal plane, and fluorescence emission from the overlapping area is prevented. Alignment accuracy of these two beams determines the image properties of the microscope. Scanning fluorescence micro-beads in the focal plane, we confirmed the beam shape and its center position for the pump and erase beams respectively. Owing to the adjustment, we achieved a point-spread function with FWHM finer than 100 nm at least, which is predicted by the theory of super-resolution microscopy.

Original languageEnglish
Pages (from-to)175-179
Number of pages5
Journale-Journal of Surface Science and Nanotechnology
Volume6
DOIs
Publication statusPublished - Jul 24 2008

Fingerprint

Immersion
numerical aperture
submerging
Lenses
Microscopy
Microscopic examination
Oils
Demonstrations
oils
lenses
microscopy
Fluorescence
Pumps
Optical transfer function
Full width at half maximum
alignment
pumps
Microscopes
fluorescence
Scanning

Keywords

  • Fluorescence-depletion
  • Laser methods
  • Nano-scale measurement
  • Scanning fluorescence microscopy
  • Super-resolution

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Materials Science (miscellaneous)

Cite this

Demonstration of super-resolution microscopy using a high numerical-aperture oil-immersion objective lens. / Iketaki, Yoshinori; Watanabe, Takeshi; Bokor, N.; Fujii, Masaaki.

In: e-Journal of Surface Science and Nanotechnology, Vol. 6, 24.07.2008, p. 175-179.

Research output: Contribution to journalArticle

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