Demonstration of light chain monotypia in B cell non-Hodgkin's lymphomas using Unfixed freeze-dried and formalin-fixed trypsinised paraffin sections

Z. Nemes, Vilmos Thomazy, G. Szeifert

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Abstract

Immunohistological light chain analysis was carried out in 55 patients with non-Hodgkin's lymphoma of B cell origin. Unfixed freeze-dried paraffin sections were used to detect surface Ig and formaldehyde-fixed trypsinised sections to detect cytoplasmic Ig. Cytoplasmic Ig was seen inconstantly in freeze-dried paraffin sections. There was complete agreement with regard to the type of light chain between freeze-dried and formaldeyde-fixed trypsinised sections. Immunohistology showed the monotypic immunoglobulin light chains in 83% of cases when unfixed freeze-dried paraffin sections were used for sIg demonstration while only in 45% of cases when formaldehyde-fixed material was used for cIg detection. The efficiency of sIg demonstration in unfixed freeze-dried paraffin sections was comparable with that based on unfixed cryostat sections or on cell suspensions when the lymphocytic lymphoma/CLL group was excluded from the evaluation. The relatively low frequency of monotypic slg positivity obtained in this group (12/19) is due to the low density of surface Ig in CLL lymphocytes.

Original languageEnglish
Pages (from-to)883-893
Number of pages11
JournalJournal of Clinical Pathology
Volume36
Issue number8
DOIs
Publication statusPublished - 1982

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B-Cell Lymphoma
Paraffin
Non-Hodgkin's Lymphoma
Formaldehyde
Light
Immunoglobulin Light Chains
B-Cell Chronic Lymphocytic Leukemia
Suspensions
B-Lymphocytes
Lymphocytes

ASJC Scopus subject areas

  • Health Policy
  • Pathology and Forensic Medicine

Cite this

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title = "Demonstration of light chain monotypia in B cell non-Hodgkin's lymphomas using Unfixed freeze-dried and formalin-fixed trypsinised paraffin sections",
abstract = "Immunohistological light chain analysis was carried out in 55 patients with non-Hodgkin's lymphoma of B cell origin. Unfixed freeze-dried paraffin sections were used to detect surface Ig and formaldehyde-fixed trypsinised sections to detect cytoplasmic Ig. Cytoplasmic Ig was seen inconstantly in freeze-dried paraffin sections. There was complete agreement with regard to the type of light chain between freeze-dried and formaldeyde-fixed trypsinised sections. Immunohistology showed the monotypic immunoglobulin light chains in 83{\%} of cases when unfixed freeze-dried paraffin sections were used for sIg demonstration while only in 45{\%} of cases when formaldehyde-fixed material was used for cIg detection. The efficiency of sIg demonstration in unfixed freeze-dried paraffin sections was comparable with that based on unfixed cryostat sections or on cell suspensions when the lymphocytic lymphoma/CLL group was excluded from the evaluation. The relatively low frequency of monotypic slg positivity obtained in this group (12/19) is due to the low density of surface Ig in CLL lymphocytes.",
author = "Z. Nemes and Vilmos Thomazy and G. Szeifert",
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T1 - Demonstration of light chain monotypia in B cell non-Hodgkin's lymphomas using Unfixed freeze-dried and formalin-fixed trypsinised paraffin sections

AU - Nemes, Z.

AU - Thomazy, Vilmos

AU - Szeifert, G.

PY - 1982

Y1 - 1982

N2 - Immunohistological light chain analysis was carried out in 55 patients with non-Hodgkin's lymphoma of B cell origin. Unfixed freeze-dried paraffin sections were used to detect surface Ig and formaldehyde-fixed trypsinised sections to detect cytoplasmic Ig. Cytoplasmic Ig was seen inconstantly in freeze-dried paraffin sections. There was complete agreement with regard to the type of light chain between freeze-dried and formaldeyde-fixed trypsinised sections. Immunohistology showed the monotypic immunoglobulin light chains in 83% of cases when unfixed freeze-dried paraffin sections were used for sIg demonstration while only in 45% of cases when formaldehyde-fixed material was used for cIg detection. The efficiency of sIg demonstration in unfixed freeze-dried paraffin sections was comparable with that based on unfixed cryostat sections or on cell suspensions when the lymphocytic lymphoma/CLL group was excluded from the evaluation. The relatively low frequency of monotypic slg positivity obtained in this group (12/19) is due to the low density of surface Ig in CLL lymphocytes.

AB - Immunohistological light chain analysis was carried out in 55 patients with non-Hodgkin's lymphoma of B cell origin. Unfixed freeze-dried paraffin sections were used to detect surface Ig and formaldehyde-fixed trypsinised sections to detect cytoplasmic Ig. Cytoplasmic Ig was seen inconstantly in freeze-dried paraffin sections. There was complete agreement with regard to the type of light chain between freeze-dried and formaldeyde-fixed trypsinised sections. Immunohistology showed the monotypic immunoglobulin light chains in 83% of cases when unfixed freeze-dried paraffin sections were used for sIg demonstration while only in 45% of cases when formaldehyde-fixed material was used for cIg detection. The efficiency of sIg demonstration in unfixed freeze-dried paraffin sections was comparable with that based on unfixed cryostat sections or on cell suspensions when the lymphocytic lymphoma/CLL group was excluded from the evaluation. The relatively low frequency of monotypic slg positivity obtained in this group (12/19) is due to the low density of surface Ig in CLL lymphocytes.

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