Demonstration of detergent-soluble antigen specific for the synaptosomal membrane-fraction isolated from the cat cerebral cortex

A. Orosz, Emilia Madarász, A. Falus, G. Adam

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Subcellular topology of an antigenic component soluble in a non-ionic detergent (Triton X-100) was investigated by means of a brain-specific immunoserum absorbed by the antigenic extracts of different organs other than that of cat cerebral cortex, as well as the water-soluble protein-antigen demonstrated in the cat brain. The Triton-soluble antigen was demonstrated in the synaptosomal fraction of the cat cerebral cortex. On subfractionating the synaptosomes, the antigen was found in the supernatant obtained by detergent treatment of the synaptosomal membrane-fraction. None of the subfractions of synaptosomes nor those of several subcellular particles other than the synaptosomal ghost-fraction reacted with the specific immunoserum in immunodiffusion tests. Data resulting from analytical ultracentrifugation, discelectrophoretic and immunological assays on the Triton-soluble antigen-preparation are reported.

Original languageEnglish
Pages (from-to)119-131
Number of pages13
JournalBrain research
Volume76
Issue number1
DOIs
Publication statusPublished - Aug 9 1974

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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