Demonstration of a Melanoma-Specific CD44 Alternative Splicing Pattern That Remains Qualitatively Stable, but Shows Quantitative Changes during Tumour Progression

Livia Raso-Barnett, Balazs Banky, Tamas Barbai, Peter Becsagh, J. Tímár, E. Rásó

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The role of CD44 in the progression of human melanoma has mostly been characterised by qualitative changes in expression of its individual variable exons. These exons however, may be expressed to form a number of molecules, the alternative splice variants of CD44, which may be structurally and functionally different. Using real-time PCR measurements with variable exon specific primers we have determined that all are expressed in human melanoma. To permit comparison between different tumours we identified a stable CD44 variable exon (CD44v) expression pattern, or CD44 'fingerprint'. This was found to remain unchanged in melanoma cell lines cultured in different matrix environments. To evaluate evolution of this fingerprint during tumour progression we established a scid mouse model, in which the pure expression pattern of metastatic primary tumours, circulating cells and metastases, non-metastatic primary tumours and lung colonies could be studied. Our analyses demonstrated, that although the melanoma CD44 fingerprint is qualitatively stable, quantitative changes are observed suggesting a possible role in tumour progression.

Original languageEnglish
Article numbere53883
JournalPLoS One
Volume8
Issue number1
DOIs
Publication statusPublished - Jan 14 2013

Fingerprint

alternative splicing
Alternative Splicing
melanoma
exons
Tumors
Exons
Melanoma
Dermatoglyphics
Demonstrations
neoplasms
Neoplasms
Circulating Neoplastic Cells
metastasis
Real-Time Polymerase Chain Reaction
quantitative polymerase chain reaction
Time measurement
animal models
lungs
cell lines
Neoplasm Metastasis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Demonstration of a Melanoma-Specific CD44 Alternative Splicing Pattern That Remains Qualitatively Stable, but Shows Quantitative Changes during Tumour Progression. / Raso-Barnett, Livia; Banky, Balazs; Barbai, Tamas; Becsagh, Peter; Tímár, J.; Rásó, E.

In: PLoS One, Vol. 8, No. 1, e53883, 14.01.2013.

Research output: Contribution to journalArticle

@article{025470b224b4422988f960177513e80a,
title = "Demonstration of a Melanoma-Specific CD44 Alternative Splicing Pattern That Remains Qualitatively Stable, but Shows Quantitative Changes during Tumour Progression",
abstract = "The role of CD44 in the progression of human melanoma has mostly been characterised by qualitative changes in expression of its individual variable exons. These exons however, may be expressed to form a number of molecules, the alternative splice variants of CD44, which may be structurally and functionally different. Using real-time PCR measurements with variable exon specific primers we have determined that all are expressed in human melanoma. To permit comparison between different tumours we identified a stable CD44 variable exon (CD44v) expression pattern, or CD44 'fingerprint'. This was found to remain unchanged in melanoma cell lines cultured in different matrix environments. To evaluate evolution of this fingerprint during tumour progression we established a scid mouse model, in which the pure expression pattern of metastatic primary tumours, circulating cells and metastases, non-metastatic primary tumours and lung colonies could be studied. Our analyses demonstrated, that although the melanoma CD44 fingerprint is qualitatively stable, quantitative changes are observed suggesting a possible role in tumour progression.",
author = "Livia Raso-Barnett and Balazs Banky and Tamas Barbai and Peter Becsagh and J. T{\'i}m{\'a}r and E. R{\'a}s{\'o}",
year = "2013",
month = "1",
day = "14",
doi = "10.1371/journal.pone.0053883",
language = "English",
volume = "8",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "1",

}

TY - JOUR

T1 - Demonstration of a Melanoma-Specific CD44 Alternative Splicing Pattern That Remains Qualitatively Stable, but Shows Quantitative Changes during Tumour Progression

AU - Raso-Barnett, Livia

AU - Banky, Balazs

AU - Barbai, Tamas

AU - Becsagh, Peter

AU - Tímár, J.

AU - Rásó, E.

PY - 2013/1/14

Y1 - 2013/1/14

N2 - The role of CD44 in the progression of human melanoma has mostly been characterised by qualitative changes in expression of its individual variable exons. These exons however, may be expressed to form a number of molecules, the alternative splice variants of CD44, which may be structurally and functionally different. Using real-time PCR measurements with variable exon specific primers we have determined that all are expressed in human melanoma. To permit comparison between different tumours we identified a stable CD44 variable exon (CD44v) expression pattern, or CD44 'fingerprint'. This was found to remain unchanged in melanoma cell lines cultured in different matrix environments. To evaluate evolution of this fingerprint during tumour progression we established a scid mouse model, in which the pure expression pattern of metastatic primary tumours, circulating cells and metastases, non-metastatic primary tumours and lung colonies could be studied. Our analyses demonstrated, that although the melanoma CD44 fingerprint is qualitatively stable, quantitative changes are observed suggesting a possible role in tumour progression.

AB - The role of CD44 in the progression of human melanoma has mostly been characterised by qualitative changes in expression of its individual variable exons. These exons however, may be expressed to form a number of molecules, the alternative splice variants of CD44, which may be structurally and functionally different. Using real-time PCR measurements with variable exon specific primers we have determined that all are expressed in human melanoma. To permit comparison between different tumours we identified a stable CD44 variable exon (CD44v) expression pattern, or CD44 'fingerprint'. This was found to remain unchanged in melanoma cell lines cultured in different matrix environments. To evaluate evolution of this fingerprint during tumour progression we established a scid mouse model, in which the pure expression pattern of metastatic primary tumours, circulating cells and metastases, non-metastatic primary tumours and lung colonies could be studied. Our analyses demonstrated, that although the melanoma CD44 fingerprint is qualitatively stable, quantitative changes are observed suggesting a possible role in tumour progression.

UR - http://www.scopus.com/inward/record.url?scp=84872305643&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872305643&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0053883

DO - 10.1371/journal.pone.0053883

M3 - Article

C2 - 23342032

AN - SCOPUS:84872305643

VL - 8

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 1

M1 - e53883

ER -