Deletions in the genomes of pseudorabies virus vaccine strains and existence of four isomers of the genomes

B. Lomniczi, M. L. Blankenship, T. Ben Porat

Research output: Contribution to journalArticle

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Abstract

As part of a study designed to identify the genes responsible for the virulence of pseudorabies virus, we have mapped the genomes of two independently derived vaccine strains (Bartha and Norden) by restriction enzyme analysis. The structures of these genomes have been compared with that of the genome of a laboratory strain previously mapped, of restriction fragments which had been cloned. The genome of the Bartha strain was found to be very similar to that of other pseudorabies virus strains, except that a deletion of approximately 2.7 x 106 daltons was found in the unique short (U(S)) region. This deletion was also observed in the genome of the Norden vaccine strain but was not observed in the genomes of any other pseudorabies virus strains that have been studied (more than 20). The genome of the Norden strain differs from that of other pseudorabies virus strains in several other respects as well. The most important difference is that in contrast to all other pseudorabies virus strains analyzed to date, which contain a type 2 herpesvirus DNA molecule (in which the U(S) region only inverts itself to the unique long [U(L)] region), the genome of the Norden strain is a type 3 molecule in which both the U(S) and the U(L) regions of the genome invert themselves, giving rise to four isomeric forms of the genome. The ability of the U(L) region to invert itself is probably related to the fact that a sequence normally present in all other pseudorabies virus strains at the end of the U(L) only is found also in inverted form at the junction of the U(L) and the internal inverted repeat in the Norden strain.

Original languageEnglish
Pages (from-to)970-979
Number of pages10
JournalJournal of Virology
Volume49
Issue number3
Publication statusPublished - 1984

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Pseudorabies Vaccines
Suid Herpesvirus 1
Suid herpesvirus 1
isomers
Genome
vaccines
genome
Vaccines
Restriction Mapping
Herpesviridae
restriction mapping

ASJC Scopus subject areas

  • Immunology

Cite this

Deletions in the genomes of pseudorabies virus vaccine strains and existence of four isomers of the genomes. / Lomniczi, B.; Blankenship, M. L.; Ben Porat, T.

In: Journal of Virology, Vol. 49, No. 3, 1984, p. 970-979.

Research output: Contribution to journalArticle

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