Deletions and structural alterations of chromosome region 89B13 from Drosophila melanogaster, by induced transposition of P{EP}EP3171 element

L. M. Magdalena, L. Savu, M. Costache, I. Kiss

Research output: Contribution to journalArticle

Abstract

Deletion mutants of the DmManf and CG14879 genes were isolated in the 89B13 region of the right arm of the 3 rd chromosome by remobilizing the P{EP}EP3171 transposon inserted between the two genes. Characterization of the 83 excision products in molecular and phenotypic terms revealed that the repair process of the DNA double strand break induced by the remobilization yielded 63% of precise excisions with completely restored nucleotide sequence and 37% imprecise excision events including 10 isolates (12%) showing homozygous lethality. To test the possibility that the lethality was caused by background mutations, the lethal alleles were crossed to the Df(3R)Exe16269 deletion. Six of them remained lethal above the deficiency, and they did not complement each other in inter se crosses either. Further molecular analysis revealed that four of these mutants are short deletions, two of them removing the 5' part of the DmManf gene while the other two partly deletes both DmManf and CG14879. In homozygous and trans-heterozygous combinations, these mutations showed polyphasic lethality during the embryonic as well as the 1 st and 2 nd larval instars. These results prove that DmManf is important for normal development and essential for viability. The mutants we recovered can be used in the future for a genetic analysis of these genes functions, especially for Manf, a potential human oncogene.

Original languageEnglish
Pages (from-to)6153-6161
Number of pages9
JournalRomanian Biotechnological Letters
Volume16
Issue number3
Publication statusPublished - May 2011

Fingerprint

transposition (genetics)
Drosophila melanogaster
Chromosomes
chromosomes
lethal genes
mutants
Genes
genes
Lethal Genes
mutation
Mutation
Chromosomes, Human, Pair 3
Double-Stranded DNA Breaks
oncogenes
Oncogenes
transposons
genetic techniques and protocols
complement
instars
viability

Keywords

  • CG14879
  • CG7013)
  • Complementation test
  • Deletion breakpoints
  • Footprint
  • Manf (ARP-like
  • P element remnant

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Agronomy and Crop Science

Cite this

Deletions and structural alterations of chromosome region 89B13 from Drosophila melanogaster, by induced transposition of P{EP}EP3171 element. / Magdalena, L. M.; Savu, L.; Costache, M.; Kiss, I.

In: Romanian Biotechnological Letters, Vol. 16, No. 3, 05.2011, p. 6153-6161.

Research output: Contribution to journalArticle

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