Deletion of the us7 and us8 genes of pseudorabies virus exerts a differential effect on the expression of early and late viral genes

Nándor Póka, Zsolt Csabai, Emese Pásti, Dóra Tombácz, Zsolt Boldogkői

Research output: Contribution to journalArticle

Abstract

The pseudorabies virus (PRV; also known as Suid herpesvirus-1) is a neurotropic herpesvirus of swine. The us7 and us8 genes of this virus encode the glycoprotein I and E membrane proteins that form a heterodimer that is known to control cell-to-cell spread in tissue culture and in animals. In this study, we investigated the effect of the deletion of the PRV us7 and us8 genes on the genome-wide transcription and DNA replication using a multi-time-point quantitative reverse transcriptase-based real-time PCR technique. Abrogation of the us7/8 gene function was found to exert a drastic but differential effect on the expression of PRV genes during lytic infection. In the mutant virus, all kinetic classes of viral genes were significantly down-regulated at the first 6 h of infection, while having been upregulated later. The level of upregulation was the highest in the immediate-early (IE) and the early (E) genes; lower in the early-late (E/L) genes; and the lowest in the late (L) genes. The relative contribution of the L transcripts to the global transcriptome became lower, while the rest of the transcripts were expressed at a higher level in the mutant than in the wild-type virus.

Original languageEnglish
Pages (from-to)603-612
Number of pages10
JournalVirus Genes
Volume53
Issue number4
DOIs
Publication statusPublished - Aug 1 2017

Fingerprint

Suid Herpesvirus 1
Viral Genes
Genes
Viruses
Herpesviridae
RNA-Directed DNA Polymerase
Infection
DNA Replication
Transcriptome
Real-Time Polymerase Chain Reaction
Glycoproteins
Membrane Proteins
Up-Regulation
Swine
Genome
Gene Expression

Keywords

  • Glycoprotein
  • Herpesvirus
  • Pseudorabies virus
  • RT-PCR
  • Transcriptome

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology

Cite this

Deletion of the us7 and us8 genes of pseudorabies virus exerts a differential effect on the expression of early and late viral genes. / Póka, Nándor; Csabai, Zsolt; Pásti, Emese; Tombácz, Dóra; Boldogkői, Zsolt.

In: Virus Genes, Vol. 53, No. 4, 01.08.2017, p. 603-612.

Research output: Contribution to journalArticle

Póka, Nándor ; Csabai, Zsolt ; Pásti, Emese ; Tombácz, Dóra ; Boldogkői, Zsolt. / Deletion of the us7 and us8 genes of pseudorabies virus exerts a differential effect on the expression of early and late viral genes. In: Virus Genes. 2017 ; Vol. 53, No. 4. pp. 603-612.
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