Deletion analysis of the flagellum-specific secretion signal in Salmonella flagellin

Noémi Kovács, Hajnalka Jankovics, Ferenc Vonderviszt

Research output: Contribution to journalArticle

Abstract

The export signal recognized by the flagellum-specific export machinery is harbored within the highly conserved 26–47 segment of the disordered N-terminal part of Salmonella flagellin. In this work, we aimed to further localize the essential part of the export signal by deletion analysis and investigated how the length of the spacer segment preceding the signal affects export efficiency. Export signal variants were attached to a reporter protein, the CCP2 domain of human C1r protein, and export efficiency of the fusion constructs was studied. Our results suggest that almost any continuous oligopeptide of 8–10 residues within the 26–47 segment can efficiently direct flagellar export if preceded by a spacer segment of at least 15 amino acids without any specific sequential requirement.

Original languageEnglish
Pages (from-to)3074-3081
Number of pages8
JournalFEBS letters
Volume592
Issue number18
DOIs
Publication statusPublished - Sep 2018

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Keywords

  • Salmonella
  • export signal
  • flagellar export
  • flagellin
  • type III secretion

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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