Delayed Recovery of Myocardial Blood Flow After Intracoronary Stem Cell Administration

Mariann Gyöngyösi, Rayyan Hemetsberger, Susanne Wolbank, Valentin Pichler, Christoph Kaun, A. Pósa, Zsolt Petrasi, Örs Petnehazy, Renate Hofer-Warbinek, Rainer de Martin, Florian Gruber, Imre Benedek, Teodora Benedek, Istvan Kovacs, Istvan Benedek, Christian A. Plass, Silvia Charwat, Gerald Maurer

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The aim of the present study was to investigate the changes in absolute myocardial blood flow (AMF) after intracoronary injections of mesenchymal SC (MSC) and compared to controls in closed-chest reperfused acute myocardial infarction (AMI) in pigs. Male MSCs, transiently transfected with Luciferase (Luc-MSC) were delivered (9:7 ± 1:2 ± 10 6) intracoronary in the open infarct-related artery one-week post-AMI in female pigs (group MSC), while saline was injected with the same injection rate in controls (group C). The AMF was measured immediately after, and 3, 12 and 24 h post-intracoronary Luc-MSC or saline injections. In vitro bioluminescence images and quantitative real-time TaqMan PCR measurements were performed to quantify the sex-mismatched MSCs. No difference between the groups was observed regarding the weight, heart rate, blood pressure and global ejection fraction 1-week post-AMI. The baseline AMF were similar in the groups (61.3 ± 15. vs 61.1 ± 12.0 ml/min). AMF was decreased significantly immediately after intracoronary MSC delivery (42.0 ± 12.4 vs 57.7 ± 15.7 ml/min p = 0.013), and remained low at 3 h (40.9 ± 13.4 vs 55.8 ± 4.9 ml/min, p = 0.004), 12 h (43.0 ± 3.7 vs 57.8 ± 5.4 ml/min, p = 0.001) with incomplete recovery at 24 h (47.2 ± 5.5 vs 62.1 ± 14.1 ml/min, p = 0.038) as compared to controls, respectively. In vitro bioluminescence displayed transfected Luc-MSCs along the proximal and mid part of the LAD, with limited number (295 ± 101 sry copied/million cardiac cells) of Y-chromosome-MSCs in the infarcted area. Intracoronary injection of SCs results in immediate decrease of AMF, with delayed recovery. The delivery of the SC into the injured myocardium might be hindered by the altered coronary pressure and flow conditions.

Original languageEnglish
Pages (from-to)616-623
Number of pages8
JournalStem Cell Reviews and Reports
Volume7
Issue number3
DOIs
Publication statusPublished - Sep 2011

Fingerprint

Stem Cells
Injections
Myocardial Infarction
Swine
Y Chromosome
Luciferases
Real-Time Polymerase Chain Reaction
Myocardium
Thorax
Arteries
Heart Rate
Blood Pressure
Pressure
Weights and Measures
Control Groups
In Vitro Techniques

Keywords

  • Blood flow
  • Catheterization
  • Imaging
  • Myocardial infarction
  • Stem cells

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology

Cite this

Gyöngyösi, M., Hemetsberger, R., Wolbank, S., Pichler, V., Kaun, C., Pósa, A., ... Maurer, G. (2011). Delayed Recovery of Myocardial Blood Flow After Intracoronary Stem Cell Administration. Stem Cell Reviews and Reports, 7(3), 616-623. https://doi.org/10.1007/s12015-010-9213-7

Delayed Recovery of Myocardial Blood Flow After Intracoronary Stem Cell Administration. / Gyöngyösi, Mariann; Hemetsberger, Rayyan; Wolbank, Susanne; Pichler, Valentin; Kaun, Christoph; Pósa, A.; Petrasi, Zsolt; Petnehazy, Örs; Hofer-Warbinek, Renate; de Martin, Rainer; Gruber, Florian; Benedek, Imre; Benedek, Teodora; Kovacs, Istvan; Benedek, Istvan; Plass, Christian A.; Charwat, Silvia; Maurer, Gerald.

In: Stem Cell Reviews and Reports, Vol. 7, No. 3, 09.2011, p. 616-623.

Research output: Contribution to journalArticle

Gyöngyösi, M, Hemetsberger, R, Wolbank, S, Pichler, V, Kaun, C, Pósa, A, Petrasi, Z, Petnehazy, Ö, Hofer-Warbinek, R, de Martin, R, Gruber, F, Benedek, I, Benedek, T, Kovacs, I, Benedek, I, Plass, CA, Charwat, S & Maurer, G 2011, 'Delayed Recovery of Myocardial Blood Flow After Intracoronary Stem Cell Administration', Stem Cell Reviews and Reports, vol. 7, no. 3, pp. 616-623. https://doi.org/10.1007/s12015-010-9213-7
Gyöngyösi, Mariann ; Hemetsberger, Rayyan ; Wolbank, Susanne ; Pichler, Valentin ; Kaun, Christoph ; Pósa, A. ; Petrasi, Zsolt ; Petnehazy, Örs ; Hofer-Warbinek, Renate ; de Martin, Rainer ; Gruber, Florian ; Benedek, Imre ; Benedek, Teodora ; Kovacs, Istvan ; Benedek, Istvan ; Plass, Christian A. ; Charwat, Silvia ; Maurer, Gerald. / Delayed Recovery of Myocardial Blood Flow After Intracoronary Stem Cell Administration. In: Stem Cell Reviews and Reports. 2011 ; Vol. 7, No. 3. pp. 616-623.
@article{94b3fc83ba264f5cbb313625b0c41c64,
title = "Delayed Recovery of Myocardial Blood Flow After Intracoronary Stem Cell Administration",
abstract = "The aim of the present study was to investigate the changes in absolute myocardial blood flow (AMF) after intracoronary injections of mesenchymal SC (MSC) and compared to controls in closed-chest reperfused acute myocardial infarction (AMI) in pigs. Male MSCs, transiently transfected with Luciferase (Luc-MSC) were delivered (9:7 ± 1:2 ± 10 6) intracoronary in the open infarct-related artery one-week post-AMI in female pigs (group MSC), while saline was injected with the same injection rate in controls (group C). The AMF was measured immediately after, and 3, 12 and 24 h post-intracoronary Luc-MSC or saline injections. In vitro bioluminescence images and quantitative real-time TaqMan PCR measurements were performed to quantify the sex-mismatched MSCs. No difference between the groups was observed regarding the weight, heart rate, blood pressure and global ejection fraction 1-week post-AMI. The baseline AMF were similar in the groups (61.3 ± 15. vs 61.1 ± 12.0 ml/min). AMF was decreased significantly immediately after intracoronary MSC delivery (42.0 ± 12.4 vs 57.7 ± 15.7 ml/min p = 0.013), and remained low at 3 h (40.9 ± 13.4 vs 55.8 ± 4.9 ml/min, p = 0.004), 12 h (43.0 ± 3.7 vs 57.8 ± 5.4 ml/min, p = 0.001) with incomplete recovery at 24 h (47.2 ± 5.5 vs 62.1 ± 14.1 ml/min, p = 0.038) as compared to controls, respectively. In vitro bioluminescence displayed transfected Luc-MSCs along the proximal and mid part of the LAD, with limited number (295 ± 101 sry copied/million cardiac cells) of Y-chromosome-MSCs in the infarcted area. Intracoronary injection of SCs results in immediate decrease of AMF, with delayed recovery. The delivery of the SC into the injured myocardium might be hindered by the altered coronary pressure and flow conditions.",
keywords = "Blood flow, Catheterization, Imaging, Myocardial infarction, Stem cells",
author = "Mariann Gy{\"o}ngy{\"o}si and Rayyan Hemetsberger and Susanne Wolbank and Valentin Pichler and Christoph Kaun and A. P{\'o}sa and Zsolt Petrasi and {\"O}rs Petnehazy and Renate Hofer-Warbinek and {de Martin}, Rainer and Florian Gruber and Imre Benedek and Teodora Benedek and Istvan Kovacs and Istvan Benedek and Plass, {Christian A.} and Silvia Charwat and Gerald Maurer",
year = "2011",
month = "9",
doi = "10.1007/s12015-010-9213-7",
language = "English",
volume = "7",
pages = "616--623",
journal = "Stem Cell Reviews",
issn = "1550-8943",
publisher = "Humana Press",
number = "3",

}

TY - JOUR

T1 - Delayed Recovery of Myocardial Blood Flow After Intracoronary Stem Cell Administration

AU - Gyöngyösi, Mariann

AU - Hemetsberger, Rayyan

AU - Wolbank, Susanne

AU - Pichler, Valentin

AU - Kaun, Christoph

AU - Pósa, A.

AU - Petrasi, Zsolt

AU - Petnehazy, Örs

AU - Hofer-Warbinek, Renate

AU - de Martin, Rainer

AU - Gruber, Florian

AU - Benedek, Imre

AU - Benedek, Teodora

AU - Kovacs, Istvan

AU - Benedek, Istvan

AU - Plass, Christian A.

AU - Charwat, Silvia

AU - Maurer, Gerald

PY - 2011/9

Y1 - 2011/9

N2 - The aim of the present study was to investigate the changes in absolute myocardial blood flow (AMF) after intracoronary injections of mesenchymal SC (MSC) and compared to controls in closed-chest reperfused acute myocardial infarction (AMI) in pigs. Male MSCs, transiently transfected with Luciferase (Luc-MSC) were delivered (9:7 ± 1:2 ± 10 6) intracoronary in the open infarct-related artery one-week post-AMI in female pigs (group MSC), while saline was injected with the same injection rate in controls (group C). The AMF was measured immediately after, and 3, 12 and 24 h post-intracoronary Luc-MSC or saline injections. In vitro bioluminescence images and quantitative real-time TaqMan PCR measurements were performed to quantify the sex-mismatched MSCs. No difference between the groups was observed regarding the weight, heart rate, blood pressure and global ejection fraction 1-week post-AMI. The baseline AMF were similar in the groups (61.3 ± 15. vs 61.1 ± 12.0 ml/min). AMF was decreased significantly immediately after intracoronary MSC delivery (42.0 ± 12.4 vs 57.7 ± 15.7 ml/min p = 0.013), and remained low at 3 h (40.9 ± 13.4 vs 55.8 ± 4.9 ml/min, p = 0.004), 12 h (43.0 ± 3.7 vs 57.8 ± 5.4 ml/min, p = 0.001) with incomplete recovery at 24 h (47.2 ± 5.5 vs 62.1 ± 14.1 ml/min, p = 0.038) as compared to controls, respectively. In vitro bioluminescence displayed transfected Luc-MSCs along the proximal and mid part of the LAD, with limited number (295 ± 101 sry copied/million cardiac cells) of Y-chromosome-MSCs in the infarcted area. Intracoronary injection of SCs results in immediate decrease of AMF, with delayed recovery. The delivery of the SC into the injured myocardium might be hindered by the altered coronary pressure and flow conditions.

AB - The aim of the present study was to investigate the changes in absolute myocardial blood flow (AMF) after intracoronary injections of mesenchymal SC (MSC) and compared to controls in closed-chest reperfused acute myocardial infarction (AMI) in pigs. Male MSCs, transiently transfected with Luciferase (Luc-MSC) were delivered (9:7 ± 1:2 ± 10 6) intracoronary in the open infarct-related artery one-week post-AMI in female pigs (group MSC), while saline was injected with the same injection rate in controls (group C). The AMF was measured immediately after, and 3, 12 and 24 h post-intracoronary Luc-MSC or saline injections. In vitro bioluminescence images and quantitative real-time TaqMan PCR measurements were performed to quantify the sex-mismatched MSCs. No difference between the groups was observed regarding the weight, heart rate, blood pressure and global ejection fraction 1-week post-AMI. The baseline AMF were similar in the groups (61.3 ± 15. vs 61.1 ± 12.0 ml/min). AMF was decreased significantly immediately after intracoronary MSC delivery (42.0 ± 12.4 vs 57.7 ± 15.7 ml/min p = 0.013), and remained low at 3 h (40.9 ± 13.4 vs 55.8 ± 4.9 ml/min, p = 0.004), 12 h (43.0 ± 3.7 vs 57.8 ± 5.4 ml/min, p = 0.001) with incomplete recovery at 24 h (47.2 ± 5.5 vs 62.1 ± 14.1 ml/min, p = 0.038) as compared to controls, respectively. In vitro bioluminescence displayed transfected Luc-MSCs along the proximal and mid part of the LAD, with limited number (295 ± 101 sry copied/million cardiac cells) of Y-chromosome-MSCs in the infarcted area. Intracoronary injection of SCs results in immediate decrease of AMF, with delayed recovery. The delivery of the SC into the injured myocardium might be hindered by the altered coronary pressure and flow conditions.

KW - Blood flow

KW - Catheterization

KW - Imaging

KW - Myocardial infarction

KW - Stem cells

UR - http://www.scopus.com/inward/record.url?scp=79959659575&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79959659575&partnerID=8YFLogxK

U2 - 10.1007/s12015-010-9213-7

DO - 10.1007/s12015-010-9213-7

M3 - Article

VL - 7

SP - 616

EP - 623

JO - Stem Cell Reviews

JF - Stem Cell Reviews

SN - 1550-8943

IS - 3

ER -