Delayed fluorescence and ultraweak light emission from isolated chloroplasts (comparison of emission spectra and concentration dependence)

Éva Hideg, Masaki Kobayashi, Humio Inaba

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The ultraweak light emission of isolated chloroplasts (Hideg and Inaba (1991) Photochem. Photobiol. 52: 137) was investigated in comparison to delayed light emission. We compared the concentration dependence and the spectral distribution of the light emitted from isolated chloroplasts stored in the dark for 10 s, 2 min (delayed light emission), 4 and 10 h (ultraweak light emission), respectively.In samples with low chlorophyll concentration, spectra of all emission phenomena were maximal at 685-695 nm, but spectra of ultraweak light, especially that of long term (10 h) emission, were broader in the 700-800 nm region than spectra of delayed light, indicating emission from a bigger variety of chlorophyll molecules.The intensity of delayed light and short term (4 h) ultraweak light exhibited a simple, saturating exponential dependence on chlorophyll concentration, while long term (10 h) ultraweak light emission was best described as a saturating exponential containing a quadratic function of the concentration. This difference suggests that long term ultraweak light emission is brought about by reactions distinct from the earlier described mechanism of electron transport related dark photoemission.

Original languageEnglish
Pages (from-to)689-693
Number of pages5
JournalPlant and Cell Physiology
Volume33
Issue number6
Publication statusPublished - Sep 1 1992

Keywords

  • Concentration dependence
  • Delayed light emission
  • Emission spectrum
  • Spinach chloroplast
  • Ultraweak light (biophoton)

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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