Deinococcus fonticola sp. nov., isolated from a radioactive thermal spring in Hungary

Judit Makk, Nóra Tünde Enyedi, E. Tóth, Dóra Anda, Attila Szabó, Tamás Felföldi, Peter Schumann, Judit Mádl-Szőnyi, A. Borsodi

Research output: Contribution to journalArticle

Abstract

A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium, designated strain FeSDHB5-19T, was isolated from a biofilm sample collected from a radioactive thermal spring (Budapest, Hungary), after exposure to 5 kGy gamma radiation. A polyphasic approach was used to study the taxonomic properties of strain FeSDHB5-19T, which had highest 16S rRNA gene sequence similarity to Deinococcus antarcticus G3-6-20T (96.5 %). The 16S rRNA gene sequence similarity to type strains of other Deinococcus species were 93.0 % or lower. The DNA G+C content of the draft genome sequence, consisting of 3.9 Mb, was 63.9 mol%. Strain FeSHDB5-19T was found to grow at temperatures of 10-32 °C (optimum, 28 °C) and pH 5-10 (pH 6.5-7.5) and tolerated up to 1.5 % NaCl (w/v) with optimum growth at 0-0.5 % NaCl. The predominant fatty acids (>10 %) were C16 : 0 and C16 : 1ω7c. The cell-wall peptidoglycan type was A3β l-Orn-Gly1-2. The whole-cell sugars were glucose and low amounts of galactose. Strain FeSDHB5-19T possessed MK-8 as the predominant respiratory quinone, typical of the genus Deinococcus. The polar lipid profile contained unidentified phosphoglycolipids and unidentified glycolipids. The isolate was found to be highly resistant to gamma (D10<8 kGy) and UV (D10~800 J m-2) radiation. According to its genotypic, phenotypic and chemotaxonomic characteristics, strain FeSDHB5-19T represents a novel species in the genus Deinococcus, for which the name Deinococcusfonticola sp. nov. is proposed. The type strain is FeSDHB5-19T (=NCAIM B.02639T=DSM 106917T).

Original languageEnglish
Pages (from-to)1724-1730
Number of pages7
JournalInternational journal of systematic and evolutionary microbiology
Volume69
Issue number6
DOIs
Publication statusPublished - Jun 1 2019

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Deinococcus
hot springs
Hungary
thermal spring
Hot Temperature
gene
new species
rRNA Genes
biofilm
sugar
glucose
fatty acid
genome
lipid
DNA
bacterium
Peptidoglycan
Glycolipids
Gamma Rays
Biofilms

Keywords

  • Deinococcus
  • gamma radiation resistance
  • radioactive thermal spring

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Deinococcus fonticola sp. nov., isolated from a radioactive thermal spring in Hungary. / Makk, Judit; Enyedi, Nóra Tünde; Tóth, E.; Anda, Dóra; Szabó, Attila; Felföldi, Tamás; Schumann, Peter; Mádl-Szőnyi, Judit; Borsodi, A.

In: International journal of systematic and evolutionary microbiology, Vol. 69, No. 6, 01.06.2019, p. 1724-1730.

Research output: Contribution to journalArticle

Makk, Judit ; Enyedi, Nóra Tünde ; Tóth, E. ; Anda, Dóra ; Szabó, Attila ; Felföldi, Tamás ; Schumann, Peter ; Mádl-Szőnyi, Judit ; Borsodi, A. / Deinococcus fonticola sp. nov., isolated from a radioactive thermal spring in Hungary. In: International journal of systematic and evolutionary microbiology. 2019 ; Vol. 69, No. 6. pp. 1724-1730.
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AU - Anda, Dóra

AU - Szabó, Attila

AU - Felföldi, Tamás

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AU - Borsodi, A.

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N2 - A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium, designated strain FeSDHB5-19T, was isolated from a biofilm sample collected from a radioactive thermal spring (Budapest, Hungary), after exposure to 5 kGy gamma radiation. A polyphasic approach was used to study the taxonomic properties of strain FeSDHB5-19T, which had highest 16S rRNA gene sequence similarity to Deinococcus antarcticus G3-6-20T (96.5 %). The 16S rRNA gene sequence similarity to type strains of other Deinococcus species were 93.0 % or lower. The DNA G+C content of the draft genome sequence, consisting of 3.9 Mb, was 63.9 mol%. Strain FeSHDB5-19T was found to grow at temperatures of 10-32 °C (optimum, 28 °C) and pH 5-10 (pH 6.5-7.5) and tolerated up to 1.5 % NaCl (w/v) with optimum growth at 0-0.5 % NaCl. The predominant fatty acids (>10 %) were C16 : 0 and C16 : 1ω7c. The cell-wall peptidoglycan type was A3β l-Orn-Gly1-2. The whole-cell sugars were glucose and low amounts of galactose. Strain FeSDHB5-19T possessed MK-8 as the predominant respiratory quinone, typical of the genus Deinococcus. The polar lipid profile contained unidentified phosphoglycolipids and unidentified glycolipids. The isolate was found to be highly resistant to gamma (D10<8 kGy) and UV (D10~800 J m-2) radiation. According to its genotypic, phenotypic and chemotaxonomic characteristics, strain FeSDHB5-19T represents a novel species in the genus Deinococcus, for which the name Deinococcusfonticola sp. nov. is proposed. The type strain is FeSDHB5-19T (=NCAIM B.02639T=DSM 106917T).

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