Deinococcus budaensis sp. Nov., a mesophilic species isolated from a biofilm sample of a hydrothermal spring cave

Judit Makk, E. Tóth, Dóra Anda, Sára Pál, Peter Schumann, A. Kovács, Judit Mádl-Szőnyi, Károly Márialigeti, A. Borsodi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Following the exposure of a biofilm sample from a hydrothermal spring cave (Gellért Hill, Budapest, Hungary) to gamma radiation, a strain designated FeSTC15-38T was isolated and studied by polyphasic taxonomic methods. The spherical-shaped cells stained Gram-negative, and were aerobic and non-motile. The pH range for growth was pH 6.0–9.0, with an optimum at pH 7.0. The temperature range for growth was 20–37 °C, with an optimum at 28 °C. Phylogenetic analysis based on the 16S rRNA gene sequence of the isolate indicated that the organism belongs to the genus Deinococcus. The highest sequence similarities appeared with Deinococcus hopiensis KR-140T (94.1%), Deinococcus aquaticus PB314T (93.3%) and Deinococcus aerophilus 5516T-11T (92.7%). The DNA G+C content of the novel strain was 68.2 mol%. The predominant fatty acids (>10%) were iso-C16: 0 and C16: 1ω7c, and the cell-wall peptidoglycan type was A3β L-Orn–Gly2-3, corroborating the assignment of the strain to the genus Deinococcus. Strain FeSTC15-38T contained MK-8 as the major menaquinone and several unidentified phospholipids, glycolipids and phosphoglycolipids. Resistance to gamma radiation (D10) of strain FeSTC15-38T was <3.0 kGy. According to phenotypic and genotypic data, strain FeSTC15-38T represents a novel species for which the name Deinococcus budaensis sp. nov. is proposed. The type strain is FeSTC15-38T (=NCAIM B.02630T=DSM 101791T).

Original languageEnglish
Pages (from-to)5345-5351
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume66
Issue number12
DOIs
Publication statusPublished - Dec 1 2016

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Deinococcus
hot springs
Biofilms
caves
biofilm
cave
phospholipid
fatty acid
Gamma Rays
phylogenetics
DNA
sampling
gene
gamma radiation
Vitamin K 2
Peptidoglycan
Hungary
temperature
Glycolipids
Base Composition

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Deinococcus budaensis sp. Nov., a mesophilic species isolated from a biofilm sample of a hydrothermal spring cave. / Makk, Judit; Tóth, E.; Anda, Dóra; Pál, Sára; Schumann, Peter; Kovács, A.; Mádl-Szőnyi, Judit; Márialigeti, Károly; Borsodi, A.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 66, No. 12, 01.12.2016, p. 5345-5351.

Research output: Contribution to journalArticle

Makk, Judit ; Tóth, E. ; Anda, Dóra ; Pál, Sára ; Schumann, Peter ; Kovács, A. ; Mádl-Szőnyi, Judit ; Márialigeti, Károly ; Borsodi, A. / Deinococcus budaensis sp. Nov., a mesophilic species isolated from a biofilm sample of a hydrothermal spring cave. In: International Journal of Systematic and Evolutionary Microbiology. 2016 ; Vol. 66, No. 12. pp. 5345-5351.
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AU - Schumann, Peter

AU - Kovács, A.

AU - Mádl-Szőnyi, Judit

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AU - Borsodi, A.

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