Dehydroepiandrosterone pretreatment alters the ischaemia/reperfusion- induced VEGF, IL-1 and IL-6 gene expression in acute renal failure

Ádám Vannay, Andrea Fekete, Róbert Langer, Tibor Tóth, Erna Sziksz, Barna Vásárhelyi, Attila J. Szabó, György Losonczy, Csaba Ádori, Anikó Gál, Tivadar Tulassay, András Szabó

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Beneficial effects of dehydroepiandrosterone (DHEA) pretreatment were reported in ischaemia/reperfusion (I/R)-induced kidney damage. Methods: To investigate the mechanism of DHEA pretreatment during renal I/R injury, the left renal pedicles of DHEA- [GDHEA; 4.0 mg/kg/day DHEA dissolved in propylene glycol (PG)] and PG-pretreated male Wistar rats (G PG) were clamped for 55 min followed by 2 (T2) and 24 h (T24) of reperfusion. Sham-operated, non-clamped animals (T 0) served as controls in both groups. Renal function, kidney morphology and interleukin 1β (IL-1β), interleukin 6 (IL-6) and vascular endothelial growth factor (VEGF) expression were determined in the kidneys of both groups. Results: Renal functional parameters and kidney structure did not differ in GDHEA versus GPG at any time point. Renal mRNA expression of IL-1β was lower at T0, while IL-6 at T2 was lower in GDHEA than in GPG.While renal VEGF mRNA expression remained unchanged, protein levels were increased at T2 and T24 compared to T0 kidneys in both groups. VEGF protein levels were lower at T2 and T24 in GDHEA than in GPG. Conclusion: We found that DHEA pretreatment alters renal IL-1β, IL-6 and VEGF synthesis. Moreover, contrary changes in VEGF mRNA and protein levels suggest that VEGF synthesis - distinct from other organs - might be primarily posttranscriptionally regulated in postischaemic rat kidneys.

Original languageEnglish
Pages (from-to)175-184
Number of pages10
JournalKidney and Blood Pressure Research
Volume32
Issue number3
DOIs
Publication statusPublished - Aug 2009

Keywords

  • Dehydroepiandrosterone
  • Interleukin 1β
  • Interleukin 6
  • Ischaemia/reperfusion
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Nephrology
  • Cardiology and Cardiovascular Medicine

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