Dehydroascorbate reduction in plant mitochondria is coupled to the respiratory electron transfer chain

A. Szarka, Nele Horemans, Zita Kovács, P. Gróf, Miklós Mayer, G. Bánhegyi

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Abstract

The reduction of dehydroascorbate (DHA) was investigated in plant mitochondria. Mitochondria isolated from Bright Yellow-2 tobacco cells were incubated with 1 mM of DHA, and the ascorbate generation was followed by high-performance liquid chromatography. Mitochondria showed clear ability to reduce DHA and to maintain a significant level of ascorbate. Ascorbate generation could be stimulated by the respiratory substrate succinate. The complex I substrate malate and the complex I inhibitor rotenone had no effect on the ascorbate generation from DHA. Similarly, the complex III inhibitor antimycin A, the alternative oxidase inhibitor salicylhydroxamic acid, and the uncoupling agent 2,4-dinitrophenol were ineffective on mitochondrial ascorbate generation both in the absence and in the presence of succinate. However, the competitive succinate dehydrogenase inhibitor malonate almost completely abolished the succinate-dependent increase in ascorbate production. The complex IV inhibitor KCN strongly stimulated ascorbate accumulation. These results together suggest that the mitochondrial respiratory chain of plant cells - presumably complex II - plays important role in the regeneration of ascorbate from its oxidized form, DHA.

Original languageEnglish
Pages (from-to)225-232
Number of pages8
JournalPhysiologia Plantarum
Volume129
Issue number1
DOIs
Publication statusPublished - Jan 2007

Fingerprint

electron transport chain
Succinic Acid
succinic acid
NADH dehydrogenase (ubiquinone)
Mitochondria
mitochondria
Electrons
succinate dehydrogenase (quinone)
Uncoupling Agents
Antimycin A
antimycin A
2,4-dinitrophenol
2,4-Dinitrophenol
Rotenone
rotenone
Succinate Dehydrogenase
Electron Transport Complex III
Plant Cells
malates
Electron Transport

ASJC Scopus subject areas

  • Plant Science

Cite this

Dehydroascorbate reduction in plant mitochondria is coupled to the respiratory electron transfer chain. / Szarka, A.; Horemans, Nele; Kovács, Zita; Gróf, P.; Mayer, Miklós; Bánhegyi, G.

In: Physiologia Plantarum, Vol. 129, No. 1, 01.2007, p. 225-232.

Research output: Contribution to journalArticle

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