Dehydroascorbate and glucose are taken up into Arabidopsis thaliana cell cultures by two distinct mechanisms

N. Horemans, A. Szarka, M. De Bock, T. Raeymaekers, G. Potters, M. Levine, G. Banhégyi, Y. Guisez

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The possible involvement of glucose (Glc) carriers in the uptake of vitamin C in plant cells is still a matter of debate. For the first time, it was shown here that plant cells exclusively take up the oxidised dehydroascorbate (DHA) form. DHA uptake is not affected by 6-bromo-6-deoxy-ascorbate, an ascorbate (ASC) analogue, specifically demonstrating ASC uptake in animal cells. There is no competition between Glc and DHA uptake. Moreover, DHA and Glc carriers respond in the opposite manner to different inhibitors (cytochalasin B, phloretin and genistein). In conclusion, the plant plasma membrane DHA carrier is distinct from the plant Glc transporters.

Original languageEnglish
Pages (from-to)2714-2718
Number of pages5
JournalFEBS letters
Volume582
Issue number18
DOIs
Publication statusPublished - Aug 6 2008

Keywords

  • Dehydroascorbate transport
  • Glucose transport
  • Plant cells
  • Plasma membrane
  • Vitamin C

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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