Degradation of RNA in rat reticulocytes. II. Relationship between RNase and its corresponding inhibitor in rat reticulocyte

Sataro Goto, Den'Ichi Mizuno

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17 Citations (Scopus)

Abstract

A latent RNase was partially purified by ammonium sulfate fractionation and Sephadex G-75 gel filtration from rat reticulocyte lysate. By mixing free RNase and its inhibitor in vitro, the latent RNase was shown to be a complex of each component. The latent RNase was activated by SH reagents, but no dissociation of the complex was observed. It was also activated by 6 m urea and 0.1 m H2SO4, and in these cases the enzyme was released as judged by gel filtration. From the results of this and precious paper, a possible mechanism of the regulation of RNase activity in rat reticulocyte is discussed.

Original languageEnglish
Pages (from-to)71-77
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume145
Issue number1
DOIs
Publication statusPublished - Jul 1971

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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