Degradation of native feathers by a novel keratinase-producing, thermophilic isolate, Brevibacillus thermoruber T1E

Zoltán Bihari, Diána Vidéki, Erzsébet Mihalik, Attila Szvetnik, Zsolt Szabó, Margit Balázs, Péter Kesseriu, I. Kiss

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Strain T1E, isolated and identified as Brevibacillus thermoruber, and evolutionally distant from the known keratinolytic isolates, proved to have feather-degrading ability. During the 7-day fermentation period, T1E consumed 10 g/l native goose feathers as the sole source of carbon and energy at 50 °C under aerobic conditions. The isolate secreted a thermostable, keratinolytic protease, which exhibited activity optimally at pH 6.5, whilst it was inhibited at alkaline pH. The keratin cleavage and catabolism resulted in the accumulation of free aspartic acid and soluble peptides with maximum values of 31.6 and 720 mg/l, respectively. The majority of the fermentation end-products were found to be small oligopeptides with an average molecular mass of 2275 Da.

Original languageEnglish
Pages (from-to)134-140
Number of pages7
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume65
Issue number1-2
Publication statusPublished - 2010

Fingerprint

Brevibacillus
Feathers
Fermentation
Geese
Degradation
Oligopeptides
Molecular mass
Keratins
Aspartic Acid
Peptide Hydrolases
Carbon
Peptides
keratinase

Keywords

  • Brevibacillus thermoruber
  • Feather
  • Keratinase

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Degradation of native feathers by a novel keratinase-producing, thermophilic isolate, Brevibacillus thermoruber T1E. / Bihari, Zoltán; Vidéki, Diána; Mihalik, Erzsébet; Szvetnik, Attila; Szabó, Zsolt; Balázs, Margit; Kesseriu, Péter; Kiss, I.

In: Zeitschrift fur Naturforschung - Section C Journal of Biosciences, Vol. 65, No. 1-2, 2010, p. 134-140.

Research output: Contribution to journalArticle

Bihari, Zoltán ; Vidéki, Diána ; Mihalik, Erzsébet ; Szvetnik, Attila ; Szabó, Zsolt ; Balázs, Margit ; Kesseriu, Péter ; Kiss, I. / Degradation of native feathers by a novel keratinase-producing, thermophilic isolate, Brevibacillus thermoruber T1E. In: Zeitschrift fur Naturforschung - Section C Journal of Biosciences. 2010 ; Vol. 65, No. 1-2. pp. 134-140.
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