Deficient interferon-γ receptor-mediated signaling in neonatal macrophages

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Abstract

Aim: To describe functional and molecular characteristics of interferon-γ (IFN-γ) activation in neonatal mononuclear phagocytes (monocytes and macrophages). Methods and Results: Exposure of cord and adult macrophages to IFN-γ gave quantitatively different results in Candida killing, as well as in release of superoxide anion (O2-). Ar concentrations of 100 U ml-1 IFN-γ, maximal increase in these functions with adult macrophages was achieved, whereas no enhancement of killing and O2- release by cord macrophages could be detected. Expression of IFN-γ receptors was comparable on cord and adult cells and specific binding of [125I]IFN-γ to cord monocytes and macrophages was even higher compared with adult cells. By flow cytometry, elements of IFN-γ receptor-mediated signaling in cord and adult monocytes and macrophages were studied. Monoclonal antibodies against the native form of the signal transducer and activator of transcription-1 (STAT-1) revealed comparable expression of this protein in cord and adult macrophages. However, STAT-1 phosphorylation in response to IFN-γ was significantly decreased in neonatal monocytes (p <0.05) and macrophages (p <0.01) compared with adult cells. Conclusion: These data suggest deficient cytokine receptor signaling in neonatal mononuclear phagocytes exposed to IFN-γ.

Original languageEnglish
Pages (from-to)117-119
Number of pages3
JournalActa Paediatrica, International Journal of Paediatrics, Supplement
Volume91
Issue number438
Publication statusPublished - 2002

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Interferon Receptors
Macrophages
Interferons
Monocytes
STAT1 Transcription Factor
Phagocytes
Cytokine Receptors
Candida
Superoxides
Flow Cytometry
Monoclonal Antibodies
Phosphorylation

Keywords

  • Cord blood
  • Interferon-γ
  • Monocytes/macrophages
  • Newborns

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

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title = "Deficient interferon-γ receptor-mediated signaling in neonatal macrophages",
abstract = "Aim: To describe functional and molecular characteristics of interferon-γ (IFN-γ) activation in neonatal mononuclear phagocytes (monocytes and macrophages). Methods and Results: Exposure of cord and adult macrophages to IFN-γ gave quantitatively different results in Candida killing, as well as in release of superoxide anion (O2-). Ar concentrations of 100 U ml-1 IFN-γ, maximal increase in these functions with adult macrophages was achieved, whereas no enhancement of killing and O2- release by cord macrophages could be detected. Expression of IFN-γ receptors was comparable on cord and adult cells and specific binding of [125I]IFN-γ to cord monocytes and macrophages was even higher compared with adult cells. By flow cytometry, elements of IFN-γ receptor-mediated signaling in cord and adult monocytes and macrophages were studied. Monoclonal antibodies against the native form of the signal transducer and activator of transcription-1 (STAT-1) revealed comparable expression of this protein in cord and adult macrophages. However, STAT-1 phosphorylation in response to IFN-γ was significantly decreased in neonatal monocytes (p <0.05) and macrophages (p <0.01) compared with adult cells. Conclusion: These data suggest deficient cytokine receptor signaling in neonatal mononuclear phagocytes exposed to IFN-γ.",
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author = "L. M{\'a}r{\'o}di",
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pages = "117--119",
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TY - JOUR

T1 - Deficient interferon-γ receptor-mediated signaling in neonatal macrophages

AU - Máródi, L.

PY - 2002

Y1 - 2002

N2 - Aim: To describe functional and molecular characteristics of interferon-γ (IFN-γ) activation in neonatal mononuclear phagocytes (monocytes and macrophages). Methods and Results: Exposure of cord and adult macrophages to IFN-γ gave quantitatively different results in Candida killing, as well as in release of superoxide anion (O2-). Ar concentrations of 100 U ml-1 IFN-γ, maximal increase in these functions with adult macrophages was achieved, whereas no enhancement of killing and O2- release by cord macrophages could be detected. Expression of IFN-γ receptors was comparable on cord and adult cells and specific binding of [125I]IFN-γ to cord monocytes and macrophages was even higher compared with adult cells. By flow cytometry, elements of IFN-γ receptor-mediated signaling in cord and adult monocytes and macrophages were studied. Monoclonal antibodies against the native form of the signal transducer and activator of transcription-1 (STAT-1) revealed comparable expression of this protein in cord and adult macrophages. However, STAT-1 phosphorylation in response to IFN-γ was significantly decreased in neonatal monocytes (p <0.05) and macrophages (p <0.01) compared with adult cells. Conclusion: These data suggest deficient cytokine receptor signaling in neonatal mononuclear phagocytes exposed to IFN-γ.

AB - Aim: To describe functional and molecular characteristics of interferon-γ (IFN-γ) activation in neonatal mononuclear phagocytes (monocytes and macrophages). Methods and Results: Exposure of cord and adult macrophages to IFN-γ gave quantitatively different results in Candida killing, as well as in release of superoxide anion (O2-). Ar concentrations of 100 U ml-1 IFN-γ, maximal increase in these functions with adult macrophages was achieved, whereas no enhancement of killing and O2- release by cord macrophages could be detected. Expression of IFN-γ receptors was comparable on cord and adult cells and specific binding of [125I]IFN-γ to cord monocytes and macrophages was even higher compared with adult cells. By flow cytometry, elements of IFN-γ receptor-mediated signaling in cord and adult monocytes and macrophages were studied. Monoclonal antibodies against the native form of the signal transducer and activator of transcription-1 (STAT-1) revealed comparable expression of this protein in cord and adult macrophages. However, STAT-1 phosphorylation in response to IFN-γ was significantly decreased in neonatal monocytes (p <0.05) and macrophages (p <0.01) compared with adult cells. Conclusion: These data suggest deficient cytokine receptor signaling in neonatal mononuclear phagocytes exposed to IFN-γ.

KW - Cord blood

KW - Interferon-γ

KW - Monocytes/macrophages

KW - Newborns

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