Deficiency of growth hormone-releasing hormone signaling is associated with sleep alterations in the dwarf rat

Ferenc Obál, Jidong Fang, Ping Taishi, Balint Kacsóh, J. Gardi, James M. Krueger

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The somatotropic axis, and particularly growth hormone-releasing hormone (GHRH), is implicated in the regulation of sleep-wake activity. To evaluate sleep in chronic somatotropic deficiency, sleep-wake activity was studied in dwarf (dw/dw) rats that are known to have a defective GHRH signaling mechanism in the pituitary and in normal Lewis rats, the parental strain of the dw/dw rats. In addition, expression of GHRH receptor (GHRH-R) mRNA in the hypothalamus/preoptic region and in the pituitary was also determined by means of reverse transcription-PCR, and GHRH content of the hypothalamus was measured. Hypothalamic/preoptic and pituitary GHRH-R mRNA levels were decreased in the dw/dw rats, indicating deficits in the central GHRHergic transmission. Hypothalamic GHRH content in dw/dw rats was also less than that found in Lewis rats. The dw/dw rats had less spontaneous nonrapid eye movement sleep (NREMS) (light and dark period) and rapid eye movement sleep (REMS) (light period) than did the control Lewis rats. After 4 hr of sleep deprivation, rebound increases in NREMS and REMS were normal in the dw/dw rat. As determined by fast Fourier analysis of the electroencephalogram (EEG), the sleep deprivation-induced enhancements in EEG slow-wave activity in the dw/dw rats were only one-half of the response in the Lewis rats. The results are compared with sleep findings previously obtained in GHRH-deficient transgenic mice. The alterations in NREMS are attributed to the defect in GHRH signaling, whereas the decreases in REMS might result from the growth hormone deficiency in the dw/dw rat.

Original languageEnglish
Pages (from-to)2912-2918
Number of pages7
JournalJournal of Neuroscience
Volume21
Issue number8
Publication statusPublished - Apr 15 2001

Fingerprint

Growth Hormone-Releasing Hormone
Sleep
REM Sleep
Eye Movements
Sleep Deprivation
Growth Hormone
Hypothalamus
Electroencephalography
Hypothalamic Hormones
Pituitary Hormone-Releasing Hormones
Light
Messenger RNA
Fourier Analysis
Transgenic Mice
Reverse Transcription

Keywords

  • Dwarf
  • GHRH receptor
  • Rats
  • Sleep
  • Sleep deprivation
  • Somatotropic axis

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Deficiency of growth hormone-releasing hormone signaling is associated with sleep alterations in the dwarf rat. / Obál, Ferenc; Fang, Jidong; Taishi, Ping; Kacsóh, Balint; Gardi, J.; Krueger, James M.

In: Journal of Neuroscience, Vol. 21, No. 8, 15.04.2001, p. 2912-2918.

Research output: Contribution to journalArticle

Obál, F, Fang, J, Taishi, P, Kacsóh, B, Gardi, J & Krueger, JM 2001, 'Deficiency of growth hormone-releasing hormone signaling is associated with sleep alterations in the dwarf rat', Journal of Neuroscience, vol. 21, no. 8, pp. 2912-2918.
Obál, Ferenc ; Fang, Jidong ; Taishi, Ping ; Kacsóh, Balint ; Gardi, J. ; Krueger, James M. / Deficiency of growth hormone-releasing hormone signaling is associated with sleep alterations in the dwarf rat. In: Journal of Neuroscience. 2001 ; Vol. 21, No. 8. pp. 2912-2918.
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