Deep sequencing analysis of viral short RNAs from an infected Pinot Noir grapevine

Vitantonio Pantaleo, Pasquale Saldarelli, Laura Miozzi, Annalisa Giampetruzzi, Andreas Gisel, Simon Moxon, Tamas Dalmay, György Bisztray, Jozsef Burgyan

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Virus-derived short interfering RNAs (vsiRNAs) isolated from grapevine V. vinifera Pinot Noir clone ENTAV 115 were analyzed by high-throughput sequencing using the Illumina Solexa platform. We identified and characterized vsiRNAs derived from grapevine field plants naturally infected with different viruses belonging to the genera Foveavirus, Maculavirus, Marafivirus and Nepovirus. These vsiRNAs were mainly of 21 and 22 nucleotides (nt) in size and were discontinuously distributed throughout Grapevine rupestris stem-pitting associated virus (GRSPaV) and Grapevine fleck virus (GFkV) genomic RNAs. Among the studied viruses, GRSPaV and GFkV vsiRNAs had a 5' terminal nucleotide bias, which differed from that described for experimental viral infections in Arabidopsis thaliana. VsiRNAs were found to originate from both genomic and antigenomic GRSPaV RNA strands, whereas with the grapevine tymoviruses GFkV and Grapevine Red Globe associated virus (GRGV), the large majority derived from the antigenomic viral strand, a feature never observed in other plant-virus interactions.

Original languageEnglish
Pages (from-to)49-56
Number of pages8
JournalVirology
Volume408
Issue number1
DOIs
Publication statusPublished - Dec 5 2010

Keywords

  • Deep sequencing
  • Grapevine
  • RNA silencing
  • Short RNAs
  • Tymovirus

ASJC Scopus subject areas

  • Virology

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  • Cite this

    Pantaleo, V., Saldarelli, P., Miozzi, L., Giampetruzzi, A., Gisel, A., Moxon, S., Dalmay, T., Bisztray, G., & Burgyan, J. (2010). Deep sequencing analysis of viral short RNAs from an infected Pinot Noir grapevine. Virology, 408(1), 49-56. https://doi.org/10.1016/j.virol.2010.09.001