In vitro fertilizációval eloállított szarvasmarha-embriók mélyhutése

Translated title of the contribution: Deep-freezing of bovine embryos derived from in vitro fertilization (IVF)

S. Cseh, Ute Kreysing, Andrea Lucas-Hahn, Heinver Niemann, Treuer Ákos

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The authors examine the effect of a direct rehydration after freezing in ethylene glycol (EG) on the survival rate of embryos derived from in vitro maturation (IVM), fertilization (IVF) and culture (IVC) and the effect of bovine cumulus cells monolayer on the viability of embryos during in vitro culture. Bovine oocytes were matured, fertilized and cultured in vitro according to the method previously described by HERRLER, LUCAS-HAHN and NIEMANN (1992). At days 6, 7, 8, 9, and 10 after in vitro insemination (Day o), good quality embryos were directly equilibrated at room temperature (20 to 25°C) with 1.8 M EG for 15 min. in a modified phosphate buffered saline (mPBS) supplemented with 10% fetal calf serum (FCS). After equilibration, embryos were cooled at 0.3°C/min. to -33°C at which point they were plunged into liquid nitrogen. Embryos were thawed by placing the straws in a 25°C water bath. The embryos were then transferred into culture medium for rehydration. For assessment of viability, embryos were cultured (36 to 48 hours) on feeder layers of bovine cumulus cells in TCM-199. Only a few, day 6 morulae survived the freezing and thawing procedure (2/33, 6.0%). High survival rates were found in day 7 and 8 blastocysts (14/19, 73.6%; 15/21, 71.4%, respectively). The survival rate of day 9 to 10 blastocysts was lower (8/20, 40%). Thus, day 7 and 8 IVMFC blastocysts seemed to be suitable for the present freezing and direct rehydration procedure (p

Original languageHungarian
Pages (from-to)69-71
Number of pages3
JournalMagyar Allatorvosok Lapja
Volume51
Issue number2
Publication statusPublished - 1996

Fingerprint

in vitro fertilization
Fertilization in Vitro
Freezing
embryo (animal)
freezing
Embryonic Structures
cattle
Fluid Therapy
Blastocyst
rehydration
blastocyst
Cumulus Cells
Ethylene Glycol
survival rate
ethylene glycol
viability
Feeder Cells
Morula
Insemination
morula

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Cseh, S., Kreysing, U., Lucas-Hahn, A., Niemann, H., & Ákos, T. (1996). In vitro fertilizációval eloállított szarvasmarha-embriók mélyhutése. Magyar Allatorvosok Lapja, 51(2), 69-71.

In vitro fertilizációval eloállított szarvasmarha-embriók mélyhutése. / Cseh, S.; Kreysing, Ute; Lucas-Hahn, Andrea; Niemann, Heinver; Ákos, Treuer.

In: Magyar Allatorvosok Lapja, Vol. 51, No. 2, 1996, p. 69-71.

Research output: Contribution to journalArticle

Cseh, S, Kreysing, U, Lucas-Hahn, A, Niemann, H & Ákos, T 1996, 'In vitro fertilizációval eloállított szarvasmarha-embriók mélyhutése', Magyar Allatorvosok Lapja, vol. 51, no. 2, pp. 69-71.
Cseh, S. ; Kreysing, Ute ; Lucas-Hahn, Andrea ; Niemann, Heinver ; Ákos, Treuer. / In vitro fertilizációval eloállított szarvasmarha-embriók mélyhutése. In: Magyar Allatorvosok Lapja. 1996 ; Vol. 51, No. 2. pp. 69-71.
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