Decreased prereceptorial glucocorticoid activating capacity in starvation due to an oxidative shift of pyridine nucleotides in the endoplasmic reticulum

Éva Kereszturi, Fanni S. Kálmán, Tamás Kardon, Miklós Csala, Gábor Bánhegyi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Redox state of pyridine nucleotides of the endoplasmic reticulum (ER) lumen was determined in different nutritional conditions. NADPH-dependent cortisone reduction and NADP+-dependent cortisol oxidation were measured in rat liver microsomes, by utilizing the luminal 11β-hydroxysteroid dehydrogenase type 1 activity. Cortisone reduction decreased, while cortisol oxidation increased during onward starvation, showing that the luminal NADPH/NADP+ ratio was substantially decreased. Cortisone or metyrapone addition caused a smaller decrease in NADPH fluorescence in microsomes from starved rats. The results demonstrate that nutrient supply is mirrored by the redox state of ER luminal pyridine nucleotides.

Original languageEnglish
Pages (from-to)4703-4708
Number of pages6
JournalFEBS letters
Volume584
Issue number22
DOIs
Publication statusPublished - Nov 19 2010

Keywords

  • 11β-Hydroxysteroid dehydrogenase type 1.
  • Endoplasmic reticulum
  • Glucocorticoid
  • NADPH
  • Redox

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Decreased prereceptorial glucocorticoid activating capacity in starvation due to an oxidative shift of pyridine nucleotides in the endoplasmic reticulum'. Together they form a unique fingerprint.

  • Cite this