Decreased prereceptorial glucocorticoid activating capacity in starvation due to an oxidative shift of pyridine nucleotides in the endoplasmic reticulum

Éva Kereszturi, Fanni S. Kálmán, T. Kardon, M. Csala, G. Bánhegyi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Redox state of pyridine nucleotides of the endoplasmic reticulum (ER) lumen was determined in different nutritional conditions. NADPH-dependent cortisone reduction and NADP+-dependent cortisol oxidation were measured in rat liver microsomes, by utilizing the luminal 11β-hydroxysteroid dehydrogenase type 1 activity. Cortisone reduction decreased, while cortisol oxidation increased during onward starvation, showing that the luminal NADPH/NADP+ ratio was substantially decreased. Cortisone or metyrapone addition caused a smaller decrease in NADPH fluorescence in microsomes from starved rats. The results demonstrate that nutrient supply is mirrored by the redox state of ER luminal pyridine nucleotides.

Original languageEnglish
Pages (from-to)4703-4708
Number of pages6
JournalFEBS Letters
Volume584
Issue number22
DOIs
Publication statusPublished - Nov 19 2010

Fingerprint

Starvation
NADP
Endoplasmic Reticulum
Glucocorticoids
Nucleotides
Cortisone
Oxidation-Reduction
Hydrocortisone
Rats
11-beta-Hydroxysteroid Dehydrogenases
Metyrapone
Oxidation
Liver Microsomes
Microsomes
Liver
Nutrients
Thermodynamic properties
Fluorescence
pyridine
Food

Keywords

  • 11β-Hydroxysteroid dehydrogenase type 1.
  • Endoplasmic reticulum
  • Glucocorticoid
  • NADPH
  • Redox

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Decreased prereceptorial glucocorticoid activating capacity in starvation due to an oxidative shift of pyridine nucleotides in the endoplasmic reticulum. / Kereszturi, Éva; Kálmán, Fanni S.; Kardon, T.; Csala, M.; Bánhegyi, G.

In: FEBS Letters, Vol. 584, No. 22, 19.11.2010, p. 4703-4708.

Research output: Contribution to journalArticle

@article{048ba546dd454ea7ab904f1c0457b8fe,
title = "Decreased prereceptorial glucocorticoid activating capacity in starvation due to an oxidative shift of pyridine nucleotides in the endoplasmic reticulum",
abstract = "Redox state of pyridine nucleotides of the endoplasmic reticulum (ER) lumen was determined in different nutritional conditions. NADPH-dependent cortisone reduction and NADP+-dependent cortisol oxidation were measured in rat liver microsomes, by utilizing the luminal 11β-hydroxysteroid dehydrogenase type 1 activity. Cortisone reduction decreased, while cortisol oxidation increased during onward starvation, showing that the luminal NADPH/NADP+ ratio was substantially decreased. Cortisone or metyrapone addition caused a smaller decrease in NADPH fluorescence in microsomes from starved rats. The results demonstrate that nutrient supply is mirrored by the redox state of ER luminal pyridine nucleotides.",
keywords = "11β-Hydroxysteroid dehydrogenase type 1., Endoplasmic reticulum, Glucocorticoid, NADPH, Redox",
author = "{\'E}va Kereszturi and K{\'a}lm{\'a}n, {Fanni S.} and T. Kardon and M. Csala and G. B{\'a}nhegyi",
year = "2010",
month = "11",
day = "19",
doi = "10.1016/j.febslet.2010.10.053",
language = "English",
volume = "584",
pages = "4703--4708",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "22",

}

TY - JOUR

T1 - Decreased prereceptorial glucocorticoid activating capacity in starvation due to an oxidative shift of pyridine nucleotides in the endoplasmic reticulum

AU - Kereszturi, Éva

AU - Kálmán, Fanni S.

AU - Kardon, T.

AU - Csala, M.

AU - Bánhegyi, G.

PY - 2010/11/19

Y1 - 2010/11/19

N2 - Redox state of pyridine nucleotides of the endoplasmic reticulum (ER) lumen was determined in different nutritional conditions. NADPH-dependent cortisone reduction and NADP+-dependent cortisol oxidation were measured in rat liver microsomes, by utilizing the luminal 11β-hydroxysteroid dehydrogenase type 1 activity. Cortisone reduction decreased, while cortisol oxidation increased during onward starvation, showing that the luminal NADPH/NADP+ ratio was substantially decreased. Cortisone or metyrapone addition caused a smaller decrease in NADPH fluorescence in microsomes from starved rats. The results demonstrate that nutrient supply is mirrored by the redox state of ER luminal pyridine nucleotides.

AB - Redox state of pyridine nucleotides of the endoplasmic reticulum (ER) lumen was determined in different nutritional conditions. NADPH-dependent cortisone reduction and NADP+-dependent cortisol oxidation were measured in rat liver microsomes, by utilizing the luminal 11β-hydroxysteroid dehydrogenase type 1 activity. Cortisone reduction decreased, while cortisol oxidation increased during onward starvation, showing that the luminal NADPH/NADP+ ratio was substantially decreased. Cortisone or metyrapone addition caused a smaller decrease in NADPH fluorescence in microsomes from starved rats. The results demonstrate that nutrient supply is mirrored by the redox state of ER luminal pyridine nucleotides.

KW - 11β-Hydroxysteroid dehydrogenase type 1.

KW - Endoplasmic reticulum

KW - Glucocorticoid

KW - NADPH

KW - Redox

UR - http://www.scopus.com/inward/record.url?scp=78249242429&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78249242429&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2010.10.053

DO - 10.1016/j.febslet.2010.10.053

M3 - Article

C2 - 21035447

AN - SCOPUS:78249242429

VL - 584

SP - 4703

EP - 4708

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 22

ER -