Decreased paraoxonase 1 (PON1) lactonase activity in hemodialyzed and renal transplanted patients. A novel cardiovascular biomarker in end-stage renal disease

Ferenc Sztanek, I. Seres, M. Harangi, Lajos Lócsey, János Padra, György J R Paragh, László Asztalos, György Parah

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background Human paraoxonase-1 (PON1) has also been described as a lactonase. Decreased PON1 lactonase activity was found to be a predictor of cardiovascular disease. Homocysteine thiolactonase activity may prevent proteins from homocysteinylation and is thought to be a protective factor against the progression of atherosclerosis. Previous studies have demonstrated decreased PON1 paraoxonase activity in hemodialyzed (HD) and renal transplant (TRX) patients; however, lactonase activity has not been investigated. We aimed to determine the paraoxonase and lactonase activities and to clarify the relationship between lactonase activity and a set of cardiovascular risk factors, such as homocysteine, cystatin C and asymmetric dimethylarginine (ADMA) levels, in HD and TRX patients and in healthy controls. Methods One hundred and eight HD and 78 TRX patients and 63 healthy controls were involved in the study. Paraoxonase and lactonase activities (paraoxon and gamma- thiobutyrolactone as substrates) were measured spectrophotometrically. ADMA level was determined with sandwich enzyme-linked immunosorbent assay. Results Both HD and TRX patients had significantly lower lactonase activities compared to the control group (P <0.05). Significantly lower paraoxonase activities were found in HD patients compared to the TRX group (P <0.05). Significant negative correlation was found between lactonase activity and ADMA level in the whole study population (P <0.001), while paraoxonase and lactonase activities showed significant positive correlation (P <0.001). Multiple regression analysis identified paraoxonase activity and homocysteine level as independent predictors of lactonase activity. Conclusion Lactonase activity is a potential new predictor of cardiovascular risk in renal failure. Measurement of lactonase activity is recommended in future studies on HD and TRX patients.

Original languageEnglish
Pages (from-to)2866-2872
Number of pages7
JournalNephrology Dialysis Transplantation
Volume27
Issue number7
DOIs
Publication statusPublished - Jul 2012

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Aryldialkylphosphatase
Chronic Kidney Failure
Biomarkers
Kidney
Homocysteine
Paraoxon
Cystatin C
Renal Insufficiency
Atherosclerosis
Cardiovascular Diseases
Enzyme-Linked Immunosorbent Assay
Regression Analysis
Transplants
Control Groups

Keywords

  • asymmetric dimethylarginine
  • chronic renal failure
  • lactonase
  • paraoxonase
  • renal transplantation

ASJC Scopus subject areas

  • Nephrology
  • Transplantation

Cite this

Decreased paraoxonase 1 (PON1) lactonase activity in hemodialyzed and renal transplanted patients. A novel cardiovascular biomarker in end-stage renal disease. / Sztanek, Ferenc; Seres, I.; Harangi, M.; Lócsey, Lajos; Padra, János; Paragh, György J R; Asztalos, László; Parah, György.

In: Nephrology Dialysis Transplantation, Vol. 27, No. 7, 07.2012, p. 2866-2872.

Research output: Contribution to journalArticle

Sztanek, Ferenc ; Seres, I. ; Harangi, M. ; Lócsey, Lajos ; Padra, János ; Paragh, György J R ; Asztalos, László ; Parah, György. / Decreased paraoxonase 1 (PON1) lactonase activity in hemodialyzed and renal transplanted patients. A novel cardiovascular biomarker in end-stage renal disease. In: Nephrology Dialysis Transplantation. 2012 ; Vol. 27, No. 7. pp. 2866-2872.
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abstract = "Background Human paraoxonase-1 (PON1) has also been described as a lactonase. Decreased PON1 lactonase activity was found to be a predictor of cardiovascular disease. Homocysteine thiolactonase activity may prevent proteins from homocysteinylation and is thought to be a protective factor against the progression of atherosclerosis. Previous studies have demonstrated decreased PON1 paraoxonase activity in hemodialyzed (HD) and renal transplant (TRX) patients; however, lactonase activity has not been investigated. We aimed to determine the paraoxonase and lactonase activities and to clarify the relationship between lactonase activity and a set of cardiovascular risk factors, such as homocysteine, cystatin C and asymmetric dimethylarginine (ADMA) levels, in HD and TRX patients and in healthy controls. Methods One hundred and eight HD and 78 TRX patients and 63 healthy controls were involved in the study. Paraoxonase and lactonase activities (paraoxon and gamma- thiobutyrolactone as substrates) were measured spectrophotometrically. ADMA level was determined with sandwich enzyme-linked immunosorbent assay. Results Both HD and TRX patients had significantly lower lactonase activities compared to the control group (P <0.05). Significantly lower paraoxonase activities were found in HD patients compared to the TRX group (P <0.05). Significant negative correlation was found between lactonase activity and ADMA level in the whole study population (P <0.001), while paraoxonase and lactonase activities showed significant positive correlation (P <0.001). Multiple regression analysis identified paraoxonase activity and homocysteine level as independent predictors of lactonase activity. Conclusion Lactonase activity is a potential new predictor of cardiovascular risk in renal failure. Measurement of lactonase activity is recommended in future studies on HD and TRX patients.",
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T1 - Decreased paraoxonase 1 (PON1) lactonase activity in hemodialyzed and renal transplanted patients. A novel cardiovascular biomarker in end-stage renal disease

AU - Sztanek, Ferenc

AU - Seres, I.

AU - Harangi, M.

AU - Lócsey, Lajos

AU - Padra, János

AU - Paragh, György J R

AU - Asztalos, László

AU - Parah, György

PY - 2012/7

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N2 - Background Human paraoxonase-1 (PON1) has also been described as a lactonase. Decreased PON1 lactonase activity was found to be a predictor of cardiovascular disease. Homocysteine thiolactonase activity may prevent proteins from homocysteinylation and is thought to be a protective factor against the progression of atherosclerosis. Previous studies have demonstrated decreased PON1 paraoxonase activity in hemodialyzed (HD) and renal transplant (TRX) patients; however, lactonase activity has not been investigated. We aimed to determine the paraoxonase and lactonase activities and to clarify the relationship between lactonase activity and a set of cardiovascular risk factors, such as homocysteine, cystatin C and asymmetric dimethylarginine (ADMA) levels, in HD and TRX patients and in healthy controls. Methods One hundred and eight HD and 78 TRX patients and 63 healthy controls were involved in the study. Paraoxonase and lactonase activities (paraoxon and gamma- thiobutyrolactone as substrates) were measured spectrophotometrically. ADMA level was determined with sandwich enzyme-linked immunosorbent assay. Results Both HD and TRX patients had significantly lower lactonase activities compared to the control group (P <0.05). Significantly lower paraoxonase activities were found in HD patients compared to the TRX group (P <0.05). Significant negative correlation was found between lactonase activity and ADMA level in the whole study population (P <0.001), while paraoxonase and lactonase activities showed significant positive correlation (P <0.001). Multiple regression analysis identified paraoxonase activity and homocysteine level as independent predictors of lactonase activity. Conclusion Lactonase activity is a potential new predictor of cardiovascular risk in renal failure. Measurement of lactonase activity is recommended in future studies on HD and TRX patients.

AB - Background Human paraoxonase-1 (PON1) has also been described as a lactonase. Decreased PON1 lactonase activity was found to be a predictor of cardiovascular disease. Homocysteine thiolactonase activity may prevent proteins from homocysteinylation and is thought to be a protective factor against the progression of atherosclerosis. Previous studies have demonstrated decreased PON1 paraoxonase activity in hemodialyzed (HD) and renal transplant (TRX) patients; however, lactonase activity has not been investigated. We aimed to determine the paraoxonase and lactonase activities and to clarify the relationship between lactonase activity and a set of cardiovascular risk factors, such as homocysteine, cystatin C and asymmetric dimethylarginine (ADMA) levels, in HD and TRX patients and in healthy controls. Methods One hundred and eight HD and 78 TRX patients and 63 healthy controls were involved in the study. Paraoxonase and lactonase activities (paraoxon and gamma- thiobutyrolactone as substrates) were measured spectrophotometrically. ADMA level was determined with sandwich enzyme-linked immunosorbent assay. Results Both HD and TRX patients had significantly lower lactonase activities compared to the control group (P <0.05). Significantly lower paraoxonase activities were found in HD patients compared to the TRX group (P <0.05). Significant negative correlation was found between lactonase activity and ADMA level in the whole study population (P <0.001), while paraoxonase and lactonase activities showed significant positive correlation (P <0.001). Multiple regression analysis identified paraoxonase activity and homocysteine level as independent predictors of lactonase activity. Conclusion Lactonase activity is a potential new predictor of cardiovascular risk in renal failure. Measurement of lactonase activity is recommended in future studies on HD and TRX patients.

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KW - chronic renal failure

KW - lactonase

KW - paraoxonase

KW - renal transplantation

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