Decreased mucosal expression of intestinal alkaline phosphatase in children with coeliac disease

Kriszta Molnár, A. Vannay, Erna Sziksz, Nóra Fanni Bánki, Hajnalka Gyorffy, A. Arató, Antal Dezsofi, G. Verès

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A major function of the enzyme intestinal alkaline phosphatase (iAP) is the detoxification of lipopolysaccharide (LPS), the ligand of Toll-like receptor 4 (TLR4). Hence, iAP has a role in the defence of maintaining intestinal barrier integrity. As intestinal barrier integrity is impaired in coeliac disease (CD), we tested the expression and localization of iAP in duodenal mucosa specimens from children with newly diagnosed CD (n=10), with CD on gluten-free diet (GFD) (n=5) and compared to those from ten healthy children. The mRNA and protein expression was determined by RT-PCR and Western blot analysis, respectively. Tissue localization of iAP and TLR4 was determined by immunofluorescence staining. iAP protein expression level was significantly lower than normal in newly diagnosed CD, while it was normalised in children on GFD. iAP and TLR4 colocalized at the epithelial surface of duodenal mucosa in each group of subjects enrolled. The finding of decreased iAP protein levels in newly diagnosed CD is consistent with its role in decreased intestinal barrier integrity. The latter may be the result of decreased LPS-detoxifying ability.

Original languageEnglish
Pages (from-to)157-161
Number of pages5
JournalVirchows Archiv
Volume460
Issue number2
DOIs
Publication statusPublished - Feb 2012

Fingerprint

Celiac Disease
Alkaline Phosphatase
Toll-Like Receptor 4
Gluten-Free Diet
Lipopolysaccharides
Mucous Membrane
Proteins
Fluorescent Antibody Technique
Western Blotting
Staining and Labeling
Ligands
Polymerase Chain Reaction
Messenger RNA
Enzymes

Keywords

  • Children
  • Coeliac disease
  • Duodenal biopsy
  • Intestinal alkaline phosphatase
  • Toll-like receptor

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Decreased mucosal expression of intestinal alkaline phosphatase in children with coeliac disease. / Molnár, Kriszta; Vannay, A.; Sziksz, Erna; Bánki, Nóra Fanni; Gyorffy, Hajnalka; Arató, A.; Dezsofi, Antal; Verès, G.

In: Virchows Archiv, Vol. 460, No. 2, 02.2012, p. 157-161.

Research output: Contribution to journalArticle

Molnár, Kriszta ; Vannay, A. ; Sziksz, Erna ; Bánki, Nóra Fanni ; Gyorffy, Hajnalka ; Arató, A. ; Dezsofi, Antal ; Verès, G. / Decreased mucosal expression of intestinal alkaline phosphatase in children with coeliac disease. In: Virchows Archiv. 2012 ; Vol. 460, No. 2. pp. 157-161.
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