De novo chromosome formations by large-scale amplification of the centromeric region of mouse chromosomes

J. Keresõ, T. Praznovszky, I. Cserpán, K. Fodor, R. Katona, E. Csonka, K. Fátyol, Gy Holló, A. Szeles, A. R. Ross, A. T. Sumner, A. A. Szalay, Gy Hadlaczky

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Abstract

Chromosomes formed de novo which originated from the centromeric region of mouse chromosome 7, have been analysed. These new chromosomes were formed by apparently similar large-scale amplification processes, and are organized into amplicons of ~30 Mb. Centromeric satellite DNA was found to be the constant component of all amplicons. Satellite DNA sequences either bordered the large euchromatic amplicons (E-type amplification), or made up the bulk of the constitutive heterochromatic amplicons (H-type amplification). Detailed analysis of a heterochromatic megachromosome formed de novo by an H-type amplification revealed that it is composed of a tandem array of 10-12 large (~30 Mb) amplicons each marked with integrated 'foreign' DNA sequences at both ends. Each amplicon is a giant palindrome, consisting of two inverted doublets of ~7.5-Mb blocks of satellite DNA. Our results indicate that the building units of the pericentric heterochromatin of mouse chromosomes are ~7.5-Mb blocks of satellite DNA flanked by non satellite sequences. We suggest that the formation de novo of various chromosome segments and chromosomes seen in different cell lines may be the result of large scale E- and H-type amplification initiated in the pericentric region of chromosomes.

Original languageEnglish
Pages (from-to)226-239
Number of pages14
JournalChromosome Research
Volume4
Issue number3
DOIs
Publication statusPublished - Jul 11 1996

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Keywords

  • Amplification
  • Centromere
  • Chromosome formation
  • Heterochromatin
  • Satellite DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Keresõ, J., Praznovszky, T., Cserpán, I., Fodor, K., Katona, R., Csonka, E., Fátyol, K., Holló, G., Szeles, A., Ross, A. R., Sumner, A. T., Szalay, A. A., & Hadlaczky, G. (1996). De novo chromosome formations by large-scale amplification of the centromeric region of mouse chromosomes. Chromosome Research, 4(3), 226-239. https://doi.org/10.1007/BF02254964