Dark septate endophytic pleosporalean genera from semiarid areas

D. G. Knapp, G. Kovács, E. Zajta, J. Z. Groenewald, P. W. Crous

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Dark septate endophytes (DSE) are distributed worldwide as root-colonising fungi, and frequent in environments with strong abiotic stress. DSE is not a taxon, but constitutes numerous fungal taxa belonging to several orders of Ascomycota. In this study we investigate three unidentified DSE lineages belonging to Pleosporales that were found previously in semiarid sandy grasslands. For molecular phylogenetic studies seven loci (ITS, partial 18S nrRNA, 28S nrRNA, actin, calmodulin, transcription-elongation factor 1-α and ß-tubulin genes) were amplified and sequenced. Based on morphology and the resulting molecular phylogeny these isolates were found to represent three novel genera within the Pleosporales, namely Aquilomyces, Flavomyces and Darksidea, with eight novel species. Molecular data revealed that monotypic Aquilomyces belongs to Morosphaeriaceae, monotypic Flavomyces represents an incertae sedis lineage related to Massarinaceae, and Darksidea, with six new species, is allied to the Lentitheciaceae. During this study we tested numerous conditions to induce sporulation, and managed for the first time to induce several DSE to form their sexual morphs.

Original languageEnglish
Pages (from-to)87-100
Number of pages14
JournalPersoonia: Molecular Phylogeny and Evolution of Fungi
Volume35
Issue number1
DOIs
Publication statusPublished - Dec 1 2015

Fingerprint

endophyte
endophytes
Pleosporales
Massarinaceae
sporulation
phylogeny
calmodulin
morphs
tubulin
Ascomycota
abiotic stress
actin
transcription (genetics)
grasslands
grassland
fungus
new species
phylogenetics
loci
fungi

Keywords

  • Dothideomycetes
  • Endophytes
  • Massarineae
  • Mating
  • Phylogeny
  • Sexual state
  • Taxonomy

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

Cite this

Dark septate endophytic pleosporalean genera from semiarid areas. / Knapp, D. G.; Kovács, G.; Zajta, E.; Groenewald, J. Z.; Crous, P. W.

In: Persoonia: Molecular Phylogeny and Evolution of Fungi, Vol. 35, No. 1, 01.12.2015, p. 87-100.

Research output: Contribution to journalArticle

Knapp, D. G. ; Kovács, G. ; Zajta, E. ; Groenewald, J. Z. ; Crous, P. W. / Dark septate endophytic pleosporalean genera from semiarid areas. In: Persoonia: Molecular Phylogeny and Evolution of Fungi. 2015 ; Vol. 35, No. 1. pp. 87-100.
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