Dark and photoreactivity of 4′-aminomethyl-4,5′,8-trimethylpsoralen with T7 phage

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Abstract

The dark and photoreactions of 4′-aminomethyl-4,5′,8-trimethylpsoralen (AMT) with T7 phage were investigated from biological and structural points of view. The dark reaction leads to the structural destabilization of the double helix of the DNA as is shown by optical melting measurements. The genotoxicity of AMT in the dark is comparable with that of known genotoxic drugs as determined by phage inactivation. The photoreaction with UVA light leads to the formation of mono- and di-adducts depending on the wavelength and dose used. Mono- and di-adducts influence DNA stability differently; biologically both types of adducts are genotoxic as measured by action spectra.

Original languageEnglish
Pages (from-to)167-178
Number of pages12
JournalJournal of Photochemistry and Photobiology, B: Biology
Volume5
Issue number2
DOIs
Publication statusPublished - Apr 15 1990

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Keywords

  • Phage photosensitization
  • action spectra.
  • dark reaction
  • optical melting

ASJC Scopus subject areas

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology Nuclear Medicine and imaging

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