Dansyl- and rhodamine-based fluorescent sensors for detecting singlet oxygen and superoxide production in plants in vivo

Csengele Barta, T. Kalai, I. Vass, K. Hideg, É. Hideg

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) play a key role in a variety of biotic and abiotic stress conditions, therefore their direct measurement in vivo is of special importance. In this study, we describe two dansyl-based ROS sensors, the singlet oxygen specific DanePy; HO-1889NH, which is reactive to both singlet oxygen and superoxide radicals and a rhodamine-based singlet oxygen sensor, HO-2941. In order to characterise the potential use of these chemosensors in plant experiments in vivo, we characterise their ROS trapping ability, fluorescence emission spectra and localisation in spinach leaves.

Original languageEnglish
Pages (from-to)149-150
Number of pages2
JournalActa Biologica Szegediensis
Volume46
Issue number3-4
Publication statusPublished - 2002

Fingerprint

Singlet Oxygen
Rhodamines
singlet oxygen
Superoxides
superoxide anion
reactive oxygen species
Reactive Oxygen Species
Sensors
Oxygen sensors
Spinacia oleracea
biotic stress
spinach
abiotic stress
trapping
Fluorescence
fluorescence
leaves
Experiments

Keywords

  • Double (fluorescent and spin)
  • Laser scanning microscopy
  • Sensors
  • Singlet oxygen
  • Superoxide radicals

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology
  • Applied Microbiology and Biotechnology
  • Neuroscience(all)

Cite this

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T1 - Dansyl- and rhodamine-based fluorescent sensors for detecting singlet oxygen and superoxide production in plants in vivo

AU - Barta, Csengele

AU - Kalai, T.

AU - Vass, I.

AU - Hideg, K.

AU - Hideg, É.

PY - 2002

Y1 - 2002

N2 - Reactive oxygen species (ROS) play a key role in a variety of biotic and abiotic stress conditions, therefore their direct measurement in vivo is of special importance. In this study, we describe two dansyl-based ROS sensors, the singlet oxygen specific DanePy; HO-1889NH, which is reactive to both singlet oxygen and superoxide radicals and a rhodamine-based singlet oxygen sensor, HO-2941. In order to characterise the potential use of these chemosensors in plant experiments in vivo, we characterise their ROS trapping ability, fluorescence emission spectra and localisation in spinach leaves.

AB - Reactive oxygen species (ROS) play a key role in a variety of biotic and abiotic stress conditions, therefore their direct measurement in vivo is of special importance. In this study, we describe two dansyl-based ROS sensors, the singlet oxygen specific DanePy; HO-1889NH, which is reactive to both singlet oxygen and superoxide radicals and a rhodamine-based singlet oxygen sensor, HO-2941. In order to characterise the potential use of these chemosensors in plant experiments in vivo, we characterise their ROS trapping ability, fluorescence emission spectra and localisation in spinach leaves.

KW - Double (fluorescent and spin)

KW - Laser scanning microscopy

KW - Sensors

KW - Singlet oxygen

KW - Superoxide radicals

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