Dalteparin sodium treatment during resuscitation inhibits hemorrhagic shock-induced leukocyte rolling and adhesion in the mesenteric microcirculation

Zsolt Balogh, A. Wolfárd, L. Szalay, Edit Orosz, János Aurél Simonka, M. Borós

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: Ischemia/reperfusion induced polymorphonuclear neutrophil leukocyte (PMN) adhesion and extravasation are pivotal for the development of post-injury multiple organ failure. We hypothesized that the deleterious microcirculatory consequences of hemorrhagic shock (HS) could be altered by low-molecular-weight heparin (LMWH) therapy. Our aim was to investigate the effects of dalteparin sodium on leukocyte-endothelial cell interactions when LMWH treatment was initiated before HS or during resuscitation. Methods: Anesthetized dogs underwent HS (40 mm Hg mean arterial pressure for 60 minutes) and resuscitation either with shed blood or with lactated Ringer's (LR) solution. LMWH or conventional heparin sodium pretreatment was administered subcutaneously before hemorrhage; or LMWH was given intravenously during resuscitation. Mesenteric post-capillary venules were observed by intravital video microscopy before and after HS, and 60 minutes, 120 minutes, and 180 minutes after resuscitation, and leukocyte rolling and firm adherence were determined. Results: HS significantly increased PMN rolling and adhesion in the mesenteric microcirculation. LMWH, but not heparin sodium pretreatment, significantly inhibited both primary and secondary interactions. LMWH treatment was also effective when initiated during resuscitation. LMWH exerted the same inhibitory effect regardless of the type of resuscitation. Conclusion: LMWH treatment during resuscitation effectively inhibits PMN rolling and adhesion.

Original languageEnglish
Pages (from-to)1062-1069
Number of pages8
JournalJournal of Trauma
Volume52
Issue number6
Publication statusPublished - 2002

Fingerprint

Dalteparin
Leukocyte Rolling
Hemorrhagic Shock
Low Molecular Weight Heparin
Microcirculation
Resuscitation
Neutrophils
Therapeutics
Heparin
Video Microscopy
Multiple Organ Failure
Venules
Cell Communication
Reperfusion
Arterial Pressure
Leukocytes
Ischemia
Endothelial Cells
Dogs
Hemorrhage

Keywords

  • Adhesion
  • Hemorrhagic shock
  • Heparin
  • Intravital video microscopy
  • Ischemia/reperfusion
  • Leukocyte

ASJC Scopus subject areas

  • Surgery

Cite this

Dalteparin sodium treatment during resuscitation inhibits hemorrhagic shock-induced leukocyte rolling and adhesion in the mesenteric microcirculation. / Balogh, Zsolt; Wolfárd, A.; Szalay, L.; Orosz, Edit; Simonka, János Aurél; Borós, M.

In: Journal of Trauma, Vol. 52, No. 6, 2002, p. 1062-1069.

Research output: Contribution to journalArticle

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AU - Szalay, L.

AU - Orosz, Edit

AU - Simonka, János Aurél

AU - Borós, M.

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