D1 protein turnover is involved in protection of Photosystem II against UV-B induced damage in the cyanobacterium Arthrospira (Spirulina) platensis

Hongyan Wu, Leyla Abasova, Otilia Cheregi, Z. Deák, Kunshan Gao, I. Vass

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

By using two strains of Arthrospira (Spirulina) platensis, an economically important filamentous cyanobacterium, we compared the impairment of PSII activity and loss of D1 protein content under UV-B radiation. Our study showed that UV-B radiation induced a gradual loss of the oxygen-evolving activity to about 56% after 180 min UV-B irradiation both in strains 439 and D-0083, which have been kept under indoor and an outdoor culturing conditions, respectively for a prolonged period of time. The loss of oxygen evolution was accelerated in both strains in the presence of lincomycin, an inhibitor of protein synthesis, and the amount of D1 protein showed a decrease comparable to that of oxygen evolution during the UV-B exposure. However, the UV-B induced loss of oxygen-evolving activity and D1 protein amount was largely prevented when A. platensis cells were exposed to UV-B irradiance supplemented with visible light. Comparison of the two strains also showed a smaller extent of D1 protein synthesis dependent PSII repair in the indoor strain. Our results show that turnover of the D1 protein is an important defense mechanism to counteract the UV-B induced damage of PSII in A. platensis, and also that visible light plays an important role in maintaining the function of PSII under simultaneous exposure to UV-B and visible light.

Original languageEnglish
Pages (from-to)320-325
Number of pages6
JournalJournal of Photochemistry and Photobiology, B: Biology
Volume104
Issue number1-2
DOIs
Publication statusPublished - Jul 2011

Fingerprint

Spirulina
Photosystem II Protein Complex
Cyanobacteria
protein synthesis
damage
proteins
Oxygen
oxygen
Proteins
Light
Radiation
impairment
Lincomycin
radiation
irradiance
Protein Synthesis Inhibitors
inhibitors
Ultraviolet Rays
irradiation
cells

Keywords

  • Arthrospira (Spirulina) platensis
  • Cyanobacterium
  • D1 protein
  • Photoinactivation
  • UV-B radiation

ASJC Scopus subject areas

  • Radiation
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Biophysics

Cite this

D1 protein turnover is involved in protection of Photosystem II against UV-B induced damage in the cyanobacterium Arthrospira (Spirulina) platensis. / Wu, Hongyan; Abasova, Leyla; Cheregi, Otilia; Deák, Z.; Gao, Kunshan; Vass, I.

In: Journal of Photochemistry and Photobiology, B: Biology, Vol. 104, No. 1-2, 07.2011, p. 320-325.

Research output: Contribution to journalArticle

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