Cytotoxicity of human lymphocytes in tissue culture

G. Szabo, G. Gasztonyi, G. Szegedi

Research output: Contribution to journalArticle

Abstract

A new 51Cr radioisotope procedure for the study of the cytotoxicity of human lymphocytes was developed. Monolayers formed by labeled amnionic cell cultures are inhibited in their adhesion to glass surfaces by cytoaggressive lymphocytes. The resulting difference in activity is measurable. The procedure is rapid, it permits 24 hr readings and the results are reproducible. The cytotoxic effect of lymphocytes of subjects with autoimmune disease and of controls were studied by this procedure. The lymphocytes of subjects being in active periods of autoimmune disease and receiving no immunosuppressive treatment caused a significant destruction of cultured human amnionic cells. The cytotoxic effect is far less extensive in periods of remission even in untreated cases. Immunosuppressive treatment results in a significant attenuation of target cell destruction. The procedure is believed to lend itself to the assessment of the degree of cell mediated immunoaggression and of the efficacy of immunosuppressive treatment in autoimmune and in heteroimmune reactions (e.g. homograft rejection). In acute experiments, administration of 6 mercaptopurine in a single 100 mg dose was followed by a total suppression of the cytotoxic activity of lymphocytes obtained from a patient with rheumatoid arthritis and 3 hr later their cytotoxicity was far less intensive than before the administration of the immunosuppressive agent.

Original languageEnglish
Pages (from-to)119-126
Number of pages8
JournalActa Medica Academiae Scientiarum Hungaricae
Volume30
Issue number1-2
Publication statusPublished - 1973

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Immunosuppressive Agents
Lymphocytes
Autoimmune Diseases
6-Mercaptopurine
Radioisotopes
Glass
Allografts
Reading
Rheumatoid Arthritis
Cell Culture Techniques
Therapeutics

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Cytotoxicity of human lymphocytes in tissue culture. / Szabo, G.; Gasztonyi, G.; Szegedi, G.

In: Acta Medica Academiae Scientiarum Hungaricae, Vol. 30, No. 1-2, 1973, p. 119-126.

Research output: Contribution to journalArticle

Szabo, G. ; Gasztonyi, G. ; Szegedi, G. / Cytotoxicity of human lymphocytes in tissue culture. In: Acta Medica Academiae Scientiarum Hungaricae. 1973 ; Vol. 30, No. 1-2. pp. 119-126.
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