Cytotoxicity and comparative binding mechanism of piperine with human serum albumin and α-1-acid glycoprotein

Daniel Pushparaju Yeggoni, Aparna Rachamallu, Monika Kallubai, Rajagopal Subramanyam

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Human serum albumin (HSA) and α-1-acid glycoprotein (AGP) (acute phase protein) are the plasma proteins in blood system which transports many drugs. To understand the pharmacological importance of piperine molecule, here, we studied the anti-inflammatory activity of piperine on mouse macrophages (RAW 264.7) cell lines, which reveals that piperine caused an increase in inhibition growth of inflammated macrophages. Further, the fluorescence maximum quenching of proteins were observed upon binding of piperine to HSA and AGP through a static quenching mechanism. The binding constants obtained from fluorescence emission were found to be Kpiperine = 5.7 ±.2 × 105 M-1 and Kpiperine = 9.3±.25 × 104 M-1 which correspond to the free energy of-7.8 and-6.71 kcal M-1at 25 °C for HSA and AGP, respectively. Further, circular dichrosim studies revealed that there is a marginal change in the secondary structural content of HSA due to partial destabilization of HSA-piperine complexes. Consequently, inference drawn from the site-specific markers (phenylbutazone, site I marker) studies to identify the binding site of HSA noticed that piperine binds at site I (IIA), which was further authenticated by molecular docking and molecular dynamic (MD) studies. The binding constants and free energy corresponding to experimental and computational analysis suggest that there are hydrophobic and hydrophilic interactions when piperine binds to HSA. Additionally, the MD studies have showed that HSA-piperine complex reaches equilibration state at around 3 ns, which prove that the HSA-piperine complex is stable in nature.

Original languageEnglish
Pages (from-to)1336-1351
Number of pages16
JournalJournal of Biomolecular Structure and Dynamics
Volume33
Issue number6
DOIs
Publication statusPublished - 2015

Keywords

  • cytotoxicity
  • drug binding
  • fluorescence quenching
  • human serum albumin
  • molecular dynamics simulation
  • piperine
  • á-1-acid glycoprotein

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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