Cytotoxic T lymphocyte (CTL) responses human cytomegalovirus pp65, IE1- Exon4, gB, pp150, and pp28 in healthy individuals: Reevaluation of prevalence of IE1-specific CTLs

Zsofia Gyulai, V. Endrész, K. Burián, Steve Pincus, Joseph Toldy, William I. Cox, Claude Meric, Stanley Plotkin, E. Gönczöl, Klara Berencsi

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Abstract

The prevalence of human cytomegalovirus (HCMV) pp65-, pp150-, IE1-exon4- , gB- and pp28-specific cytotoxic T lymphocyte (CTL) responses was compared among 34 healthy individuals, grouped by neutralizing antibody titers. Moderately and highly seropositive donors showed predominantly pp65- and IE1- exon4-specific CTL responses (92% and 76% of the donors, respectively), with similar precursor frequencies in the 2 donors tested. In addition, highly seropositive and a few moderately seropositive donors showed CTL responses to gB and pp150 (33% and 30% of the donors, respectively). No individual recognized pp28 as a target in the CTL assay. Phenotypic analysis revealed a mixed effector population of CD4+ and CD8+ (1 donor) or only CD8+ cells for pp65-specific effectors (2 donors). IE1-exon4 and pp150-specific effectors were CD8+ (2 donors and 1 donor, respectively), whereas gB- specific CTLs were CD4+ (1 donor). These data may help to design a cellular immunity-based vaccine effective against HCMV diseases.

Original languageEnglish
Pages (from-to)1537-1546
Number of pages10
JournalJournal of Infectious Diseases
Volume181
Issue number5
DOIs
Publication statusPublished - 2000

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Cytotoxic T-Lymphocytes
Cytomegalovirus
Neutralizing Antibodies
Cellular Immunity
Vaccines
Population

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

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Cytotoxic T lymphocyte (CTL) responses human cytomegalovirus pp65, IE1- Exon4, gB, pp150, and pp28 in healthy individuals : Reevaluation of prevalence of IE1-specific CTLs. / Gyulai, Zsofia; Endrész, V.; Burián, K.; Pincus, Steve; Toldy, Joseph; Cox, William I.; Meric, Claude; Plotkin, Stanley; Gönczöl, E.; Berencsi, Klara.

In: Journal of Infectious Diseases, Vol. 181, No. 5, 2000, p. 1537-1546.

Research output: Contribution to journalArticle

Gyulai, Zsofia ; Endrész, V. ; Burián, K. ; Pincus, Steve ; Toldy, Joseph ; Cox, William I. ; Meric, Claude ; Plotkin, Stanley ; Gönczöl, E. ; Berencsi, Klara. / Cytotoxic T lymphocyte (CTL) responses human cytomegalovirus pp65, IE1- Exon4, gB, pp150, and pp28 in healthy individuals : Reevaluation of prevalence of IE1-specific CTLs. In: Journal of Infectious Diseases. 2000 ; Vol. 181, No. 5. pp. 1537-1546.
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