Cytotoxic and multidrug resistance reversal activity of a vegetable, 'Anastasia Red', a variety of sweet pepper

Noboru Motohashi, Hidetsugu Wakabayashi, Teruo Kurihara, Yuko Takada, Shichiro Maruyama, Hiroshi Sakagami, Hideki Nakashima, Satoru Tani, Yoshiaki Shirataki, Masami Kawase, Kristina Wolfard, Joseph Moinár

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The vegetable, Anastasia Red, Capsicum annuum L. var. angulosum Mill. (Solanaceae) was successively extracted with hexane, acetone, methanol and 70% methanol, and the extracts were further separated into a total of 21 fractions by silica gel or octadecylsilane (ODS) column chromatography. The biological activities of extracts and fractions were determined. These extracts showed relatively higher cytotoxic activity against two human oral tumor cell lines (HSC-2, HSG) than against normal human gingival fibroblasts (HGF), suggesting a tumor-specific cytotoxic activity. The cytotoxic activity of these extracts was enhanced by fractionation on silica gel [H2, A2, M1-M3] or ODS column chromatography [70M]. Several fractions [H2, H4, H5, A1, A2, A3, A5, A6, A7, M2] reversed the multidrug resistance (MDR) phenotype with L5178 mouse lymphoma T cells, more efficiently than (±)-verapamil. The extracts and fractions did not show any detectable anti-human immunodeficiency virus (HIV) or anti-Helicobacter pylori activity. Thus, this study suggests the effective and selective antitumor potential of 'Anastasia Red' of sweet pepper for further phytochemical and biological investigation.

Original languageEnglish
Pages (from-to)348-352
Number of pages5
JournalPhytotherapy Research
Issue number4
Publication statusPublished - Apr 1 2003



  • Anti-HIV activity
  • Anti-Helicobacter pylori activity
  • Capsicum annuum L. var. angulosum Mill.
  • Cytotoxic activity
  • Extracts and fractions
  • Multidrug resistance reversal activity

ASJC Scopus subject areas

  • Pharmacology

Cite this

Motohashi, N., Wakabayashi, H., Kurihara, T., Takada, Y., Maruyama, S., Sakagami, H., Nakashima, H., Tani, S., Shirataki, Y., Kawase, M., Wolfard, K., & Moinár, J. (2003). Cytotoxic and multidrug resistance reversal activity of a vegetable, 'Anastasia Red', a variety of sweet pepper. Phytotherapy Research, 17(4), 348-352.