Cytotoxic and multidrug resistance reversal activity of a vegetable, 'Anastasia Red', a variety of sweet pepper

Noboru Motohashi, Hidetsugu Wakabayashi, Teruo Kurihara, Yuko Takada, Shichiro Maruyama, Hiroshi Sakagami, Hideki Nakashima, Satoru Tani, Yoshiaki Shirataki, Masami Kawase, Kristina Wolfard, J. Molnár

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The vegetable, Anastasia Red, Capsicum annuum L. var. angulosum Mill. (Solanaceae) was successively extracted with hexane, acetone, methanol and 70% methanol, and the extracts were further separated into a total of 21 fractions by silica gel or octadecylsilane (ODS) column chromatography. The biological activities of extracts and fractions were determined. These extracts showed relatively higher cytotoxic activity against two human oral tumor cell lines (HSC-2, HSG) than against normal human gingival fibroblasts (HGF), suggesting a tumor-specific cytotoxic activity. The cytotoxic activity of these extracts was enhanced by fractionation on silica gel [H2, A2, M1-M3] or ODS column chromatography [70M]. Several fractions [H2, H4, H5, A1, A2, A3, A5, A6, A7, M2] reversed the multidrug resistance (MDR) phenotype with L5178 mouse lymphoma T cells, more efficiently than (±)-verapamil. The extracts and fractions did not show any detectable anti-human immunodeficiency virus (HIV) or anti-Helicobacter pylori activity. Thus, this study suggests the effective and selective antitumor potential of 'Anastasia Red' of sweet pepper for further phytochemical and biological investigation.

Original languageEnglish
Pages (from-to)348-352
Number of pages5
JournalPhytotherapy Research
Volume17
Issue number4
DOIs
Publication statusPublished - Apr 2003

Fingerprint

Capsicum
Column chromatography
Silica Gel
Vegetables
Multiple Drug Resistance
Methanol
Tumors
varespladib methyl
T-cells
Chromatography
Phytochemicals
Hexanes
Fibroblasts
Fractionation
Verapamil
Acetone
Bioactivity
Viruses
Solanaceae
T-Cell Lymphoma

Keywords

  • Anti-Helicobacter pylori activity
  • Anti-HIV activity
  • Capsicum annuum L. var. angulosum Mill.
  • Cytotoxic activity
  • Extracts and fractions
  • Multidrug resistance reversal activity

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Cytotoxic and multidrug resistance reversal activity of a vegetable, 'Anastasia Red', a variety of sweet pepper. / Motohashi, Noboru; Wakabayashi, Hidetsugu; Kurihara, Teruo; Takada, Yuko; Maruyama, Shichiro; Sakagami, Hiroshi; Nakashima, Hideki; Tani, Satoru; Shirataki, Yoshiaki; Kawase, Masami; Wolfard, Kristina; Molnár, J.

In: Phytotherapy Research, Vol. 17, No. 4, 04.2003, p. 348-352.

Research output: Contribution to journalArticle

Motohashi, N, Wakabayashi, H, Kurihara, T, Takada, Y, Maruyama, S, Sakagami, H, Nakashima, H, Tani, S, Shirataki, Y, Kawase, M, Wolfard, K & Molnár, J 2003, 'Cytotoxic and multidrug resistance reversal activity of a vegetable, 'Anastasia Red', a variety of sweet pepper', Phytotherapy Research, vol. 17, no. 4, pp. 348-352. https://doi.org/10.1002/ptr.1144
Motohashi, Noboru ; Wakabayashi, Hidetsugu ; Kurihara, Teruo ; Takada, Yuko ; Maruyama, Shichiro ; Sakagami, Hiroshi ; Nakashima, Hideki ; Tani, Satoru ; Shirataki, Yoshiaki ; Kawase, Masami ; Wolfard, Kristina ; Molnár, J. / Cytotoxic and multidrug resistance reversal activity of a vegetable, 'Anastasia Red', a variety of sweet pepper. In: Phytotherapy Research. 2003 ; Vol. 17, No. 4. pp. 348-352.
@article{13d2da12335e446a939426c0939679bd,
title = "Cytotoxic and multidrug resistance reversal activity of a vegetable, 'Anastasia Red', a variety of sweet pepper",
abstract = "The vegetable, Anastasia Red, Capsicum annuum L. var. angulosum Mill. (Solanaceae) was successively extracted with hexane, acetone, methanol and 70{\%} methanol, and the extracts were further separated into a total of 21 fractions by silica gel or octadecylsilane (ODS) column chromatography. The biological activities of extracts and fractions were determined. These extracts showed relatively higher cytotoxic activity against two human oral tumor cell lines (HSC-2, HSG) than against normal human gingival fibroblasts (HGF), suggesting a tumor-specific cytotoxic activity. The cytotoxic activity of these extracts was enhanced by fractionation on silica gel [H2, A2, M1-M3] or ODS column chromatography [70M]. Several fractions [H2, H4, H5, A1, A2, A3, A5, A6, A7, M2] reversed the multidrug resistance (MDR) phenotype with L5178 mouse lymphoma T cells, more efficiently than (±)-verapamil. The extracts and fractions did not show any detectable anti-human immunodeficiency virus (HIV) or anti-Helicobacter pylori activity. Thus, this study suggests the effective and selective antitumor potential of 'Anastasia Red' of sweet pepper for further phytochemical and biological investigation.",
keywords = "Anti-Helicobacter pylori activity, Anti-HIV activity, Capsicum annuum L. var. angulosum Mill., Cytotoxic activity, Extracts and fractions, Multidrug resistance reversal activity",
author = "Noboru Motohashi and Hidetsugu Wakabayashi and Teruo Kurihara and Yuko Takada and Shichiro Maruyama and Hiroshi Sakagami and Hideki Nakashima and Satoru Tani and Yoshiaki Shirataki and Masami Kawase and Kristina Wolfard and J. Moln{\'a}r",
year = "2003",
month = "4",
doi = "10.1002/ptr.1144",
language = "English",
volume = "17",
pages = "348--352",
journal = "Phytotherapy Research",
issn = "0951-418X",
publisher = "John Wiley and Sons Ltd",
number = "4",

}

TY - JOUR

T1 - Cytotoxic and multidrug resistance reversal activity of a vegetable, 'Anastasia Red', a variety of sweet pepper

AU - Motohashi, Noboru

AU - Wakabayashi, Hidetsugu

AU - Kurihara, Teruo

AU - Takada, Yuko

AU - Maruyama, Shichiro

AU - Sakagami, Hiroshi

AU - Nakashima, Hideki

AU - Tani, Satoru

AU - Shirataki, Yoshiaki

AU - Kawase, Masami

AU - Wolfard, Kristina

AU - Molnár, J.

PY - 2003/4

Y1 - 2003/4

N2 - The vegetable, Anastasia Red, Capsicum annuum L. var. angulosum Mill. (Solanaceae) was successively extracted with hexane, acetone, methanol and 70% methanol, and the extracts were further separated into a total of 21 fractions by silica gel or octadecylsilane (ODS) column chromatography. The biological activities of extracts and fractions were determined. These extracts showed relatively higher cytotoxic activity against two human oral tumor cell lines (HSC-2, HSG) than against normal human gingival fibroblasts (HGF), suggesting a tumor-specific cytotoxic activity. The cytotoxic activity of these extracts was enhanced by fractionation on silica gel [H2, A2, M1-M3] or ODS column chromatography [70M]. Several fractions [H2, H4, H5, A1, A2, A3, A5, A6, A7, M2] reversed the multidrug resistance (MDR) phenotype with L5178 mouse lymphoma T cells, more efficiently than (±)-verapamil. The extracts and fractions did not show any detectable anti-human immunodeficiency virus (HIV) or anti-Helicobacter pylori activity. Thus, this study suggests the effective and selective antitumor potential of 'Anastasia Red' of sweet pepper for further phytochemical and biological investigation.

AB - The vegetable, Anastasia Red, Capsicum annuum L. var. angulosum Mill. (Solanaceae) was successively extracted with hexane, acetone, methanol and 70% methanol, and the extracts were further separated into a total of 21 fractions by silica gel or octadecylsilane (ODS) column chromatography. The biological activities of extracts and fractions were determined. These extracts showed relatively higher cytotoxic activity against two human oral tumor cell lines (HSC-2, HSG) than against normal human gingival fibroblasts (HGF), suggesting a tumor-specific cytotoxic activity. The cytotoxic activity of these extracts was enhanced by fractionation on silica gel [H2, A2, M1-M3] or ODS column chromatography [70M]. Several fractions [H2, H4, H5, A1, A2, A3, A5, A6, A7, M2] reversed the multidrug resistance (MDR) phenotype with L5178 mouse lymphoma T cells, more efficiently than (±)-verapamil. The extracts and fractions did not show any detectable anti-human immunodeficiency virus (HIV) or anti-Helicobacter pylori activity. Thus, this study suggests the effective and selective antitumor potential of 'Anastasia Red' of sweet pepper for further phytochemical and biological investigation.

KW - Anti-Helicobacter pylori activity

KW - Anti-HIV activity

KW - Capsicum annuum L. var. angulosum Mill.

KW - Cytotoxic activity

KW - Extracts and fractions

KW - Multidrug resistance reversal activity

UR - http://www.scopus.com/inward/record.url?scp=0038221269&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038221269&partnerID=8YFLogxK

U2 - 10.1002/ptr.1144

DO - 10.1002/ptr.1144

M3 - Article

VL - 17

SP - 348

EP - 352

JO - Phytotherapy Research

JF - Phytotherapy Research

SN - 0951-418X

IS - 4

ER -