Cytosolic calcium changes affect the incidence of early afterdepolarizations in canine ventricular myocytes

Balázs Horváth, Bence Hegyi, Kornél Kistamás, Krisztina Váczi, Tamás Bányász, János Magyar, Norbert Szentandrássy, Péter P. Nánási

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Abstract

This study was designed to investigate the influence of cytosolic Ca2+ levels ([Ca2+]i) on action potential duration (APD) and on the incidence of early afterdepolarizations (EADs) in canine ventricular cardiomyocytes. Action potentials (AP) of isolated cells were recorded using conventional sharp microelectrodes, and the concomitant [Ca2+]i was monitored with the fluorescent dye Fura-2. EADs were evoked at a 0.2 Hz pacing rate by inhibiting the rapid delayed rectifier K+ current with dofetilide, by activating the late sodium current with veratridine, or by activating the L-type calcium current with BAY K8644. These interventions progressively prolonged the AP and resulted in initiation of EADs. Reducing [Ca2+]i by application of the cell-permeant Ca2+ chelator BAPTA-AM lengthened the AP at 1.0 Hz if it was applied alone, in the presence of veratridine, or in the presence of BAY K8644. However, BAPTA-AM shortened the AP if the cells were pretreated with dofetilide. The incidence of the evoked EADs was strongly reduced by BAPTA-AM in dofetilide, moderately reduced in veratridine, whereas EAD incidence was increased by BAPTA-AM in the presence of BAY K8644. Based on these experimental data, changes in [Ca2+]i have marked effects on APD as well as on the incidence of EADs; however, the underlying mechanisms may be different, depending on the mechanism of EAD generation. As a consequence, reduction of [Ca2+]i may eliminate EADs under some, but not all, experimental conditions.

Original languageEnglish
Pages (from-to)527-534
Number of pages8
JournalCanadian journal of physiology and pharmacology
Volume93
Issue number7
DOIs
Publication statusPublished - Mar 11 2015

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Keywords

  • Action potential duration
  • Arrhythmogenesis
  • Ca chelators
  • Canine myocytes
  • Early afterdepolarizations
  • Electrophysiology
  • Fluorescent Ca indicator dyes
  • Intracellular Ca levels

ASJC Scopus subject areas

  • Physiology
  • Pharmacology
  • Physiology (medical)

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