Cytoprotective effect of (-)-deprenyl, (-)desmethyl-deprenyl and (-)deprenyl-N-oxide on glutathione depleted A-2058 melanoma cells

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4 Citations (Scopus)

Abstract

Postulated cytoprotective action of (-)-deprenyl (D), (-)-desmethyl- deprenyl (DD) and (-)-deprenyl-N-oxide (DNO) on l-buthionine-(S,R)-sulfoximine (BSO) toxicity was investigated using in vitro cultures of serum-deprived A-2058 melanoma cells. BSO (10 μM/l) decreased viable cell number and mitotic rate, while increased the apoptotic index. D and both of its metabolites, given together with BSO in the concentration of 50 μM/l, mitigated cell loss and decreased the apoptotic ratio. DD was the most effective compound in decreasing apoptotic activity, while DNO stabilized the cell number on control level and increasing the ratio of mitotic cells above the only serum-deprived control. Surveillance on mitochondrial membrane stability and antioxidant properties may play an important role in these processes.

Original languageEnglish
Pages (from-to)695-698
Number of pages4
JournalJournal of Neural Transmission
Volume117
Issue number6
DOIs
Publication statusPublished - Jun 2010

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Selegiline
Glutathione
Melanoma
Cell Count
Mitochondrial Membranes
Serum
Antioxidants
deprenyl-N-oxide

Keywords

  • (-)-Deprenyl
  • Glutathione depletion
  • l-buthionine-(S,R)-sulfoximine
  • Melanoma cells
  • Tissue culture

ASJC Scopus subject areas

  • Biological Psychiatry
  • Neurology
  • Clinical Neurology
  • Psychiatry and Mental health

Cite this

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title = "Cytoprotective effect of (-)-deprenyl, (-)desmethyl-deprenyl and (-)deprenyl-N-oxide on glutathione depleted A-2058 melanoma cells",
abstract = "Postulated cytoprotective action of (-)-deprenyl (D), (-)-desmethyl- deprenyl (DD) and (-)-deprenyl-N-oxide (DNO) on l-buthionine-(S,R)-sulfoximine (BSO) toxicity was investigated using in vitro cultures of serum-deprived A-2058 melanoma cells. BSO (10 μM/l) decreased viable cell number and mitotic rate, while increased the apoptotic index. D and both of its metabolites, given together with BSO in the concentration of 50 μM/l, mitigated cell loss and decreased the apoptotic ratio. DD was the most effective compound in decreasing apoptotic activity, while DNO stabilized the cell number on control level and increasing the ratio of mitotic cells above the only serum-deprived control. Surveillance on mitochondrial membrane stability and antioxidant properties may play an important role in these processes.",
keywords = "(-)-Deprenyl, Glutathione depletion, l-buthionine-(S,R)-sulfoximine, Melanoma cells, Tissue culture",
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T1 - Cytoprotective effect of (-)-deprenyl, (-)desmethyl-deprenyl and (-)deprenyl-N-oxide on glutathione depleted A-2058 melanoma cells

AU - Szende, B.

AU - Barna, G.

AU - Magyar, K.

PY - 2010/6

Y1 - 2010/6

N2 - Postulated cytoprotective action of (-)-deprenyl (D), (-)-desmethyl- deprenyl (DD) and (-)-deprenyl-N-oxide (DNO) on l-buthionine-(S,R)-sulfoximine (BSO) toxicity was investigated using in vitro cultures of serum-deprived A-2058 melanoma cells. BSO (10 μM/l) decreased viable cell number and mitotic rate, while increased the apoptotic index. D and both of its metabolites, given together with BSO in the concentration of 50 μM/l, mitigated cell loss and decreased the apoptotic ratio. DD was the most effective compound in decreasing apoptotic activity, while DNO stabilized the cell number on control level and increasing the ratio of mitotic cells above the only serum-deprived control. Surveillance on mitochondrial membrane stability and antioxidant properties may play an important role in these processes.

AB - Postulated cytoprotective action of (-)-deprenyl (D), (-)-desmethyl- deprenyl (DD) and (-)-deprenyl-N-oxide (DNO) on l-buthionine-(S,R)-sulfoximine (BSO) toxicity was investigated using in vitro cultures of serum-deprived A-2058 melanoma cells. BSO (10 μM/l) decreased viable cell number and mitotic rate, while increased the apoptotic index. D and both of its metabolites, given together with BSO in the concentration of 50 μM/l, mitigated cell loss and decreased the apoptotic ratio. DD was the most effective compound in decreasing apoptotic activity, while DNO stabilized the cell number on control level and increasing the ratio of mitotic cells above the only serum-deprived control. Surveillance on mitochondrial membrane stability and antioxidant properties may play an important role in these processes.

KW - (-)-Deprenyl

KW - Glutathione depletion

KW - l-buthionine-(S,R)-sulfoximine

KW - Melanoma cells

KW - Tissue culture

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