Cytoplasmic sequestration phenomena in smooth muscle cells of kidney resistance vessels and epithelioid cells of the juxtaglomerular apparatus

R. Metz, R. Nobiling, M. Harlacher, L. Rosivall, R. Taugner

Research output: Contribution to journalArticle

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Abstract

The occurrence of vacuoles in cells of contractile tissues and especially in media cells of resistance vessels has been known for quite some time. Recently, it has been widely accepted that these vacuoles, characteristically lined by a double membrane, result from herniation of one vascular smooth muscle cell into the other as a result of vasoconstriction. In our electronmicroscopic investigations we found double membrane-bounded vacuoles not only in kidney resistance vessels of rats and mice under conditions of vasoconstriction, but also in control animals and animals with maximal renal vasodilation. Part of our observations are compatible with the assumption that such vacuoles arise from a damage of club-shaped, musculo-muscular contacts due to shape changes of media cells during maximal vasoconstriction or vasodilation. However, serial thin sectioning revealed that some of the cytoplasmic vacuoles have no connections with neighbouring cells. This finding and various parallels to the generation of autophagic vacuoles indicate that the so-called herniations may also represent demarcations of large cytoplasmic areas within an individual cell. Irrespective of the origin of these vacuoles, their contents show different stages of deterioration. At later stages, the vacuoles appear to be adjacent, with only one membrane, to the extracellular space, into which they are believed to discharge finally. Cytoplasmic vacuolization has not only been observed in smooth muscle cells, but also in juxtaglomerular epithelioid cells of the afferent arteriole. Here the vacuoles - besides other organelles - also contain secretory granules; it is therefore proposed that autophagic phenomena with final extrusion of cytoplasmic material may be involved in the programmed down-regulation of the granular renin store following inhibition of renin synthesis and secretion.

Original languageEnglish
Pages (from-to)583-589
Number of pages7
JournalVirchows Archiv A Pathological Anatomy and Histopathology
Volume412
Issue number6
DOIs
Publication statusPublished - Nov 1988

Fingerprint

Juxtaglomerular Apparatus
Epithelioid Cells
Vacuoles
Smooth Muscle Myocytes
Kidney
Vasoconstriction
Renin
Vasodilation
Membranes
Microtomy
Secretory Vesicles
Extracellular Space
Arterioles
Vascular Smooth Muscle
Organelles
Down-Regulation

Keywords

  • Cytoplasmic vacuoles
  • Epithelioid cells
  • Juxtaglomerular apparatus
  • Preglomerular arterioles
  • Vascular smooth muscle cells

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Anatomy

Cite this

Cytoplasmic sequestration phenomena in smooth muscle cells of kidney resistance vessels and epithelioid cells of the juxtaglomerular apparatus. / Metz, R.; Nobiling, R.; Harlacher, M.; Rosivall, L.; Taugner, R.

In: Virchows Archiv A Pathological Anatomy and Histopathology, Vol. 412, No. 6, 11.1988, p. 583-589.

Research output: Contribution to journalArticle

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