Cytophotometric measurement of basic proteins by the heparin alcian blue method (Hungarian)

E. Pokorny, Z. Szentirmay, J. Sugar

Research output: Contribution to journalArticle

Abstract

Reaction of heparin alcian blue (HAB) suitable for histochemical demonstration of basic proteins can be inhibited by the van Slyke desamination. This method may also be used for quantitative cytochemical investigations. Absorptive binding disturbing the cytophotometric measurement was not noticed. In tumor cells treated by vincristine the content of basic proteins increases. This phenomenon can be proved by reaction HAB. Using the same specimen Feulgen's reaction may also be carried out.

Original languageEnglish
Pages (from-to)171-176
Number of pages6
JournalMorphologiai es Igazsagugyi Orvosi Szemle
Volume14
Issue number3
Publication statusPublished - Jan 1 1974

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ASJC Scopus subject areas

  • Medicine(all)

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