Cytophotometric determination of basic proteins with heparin-alcian blue method

Éva Pokorny, Zoltán Szentirmay, János Sugár

Research output: Contribution to journalArticle

Abstract

For the quantitative cyfcochemical determination of basic proteins, Heparin-Alcian Blue (HAB-was found to be a more suitable staining procedure than the generally used alcalic Fast green reaction. HAB is able to stain all the histone fractions, but there is no staining following deamination (gelelectrophoretic examinations) and no aspecific staining (Millipore filter model). In Ehrlich ascites tumour cells, changes in the Feulgen-DNA. content is followed by the proportional changes of HAB-basic protein content. After HAB reaction, the Feulgen reaction can be performed on the same slide.

Original languageEnglish
Pages (from-to)203-209
Number of pages7
JournalActa histochemica
Volume58
Issue number2
DOIs
Publication statusPublished - Jan 1 1977

ASJC Scopus subject areas

  • Histology
  • Cell Biology

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