Cytophotometric determination of basic proteins with heparin alcian blue method

E. Pokorny, Z. Szentirmay, J. Sugar

Research output: Contribution to journalArticle

Abstract

For the quantitative cytochemical determination of basic proteins, heparin-alcian blue (HAB) was found to be a more suitable staining procedure than the generally used fast green reaction. HAB is able to stain all the histone fractions, but there is no staining following deamination (gel electrophoretic examinations) and no aspecific staining (Millipore filter model). In Ehrlich ascites tumor cells, changes in the Feulgen-DNA content is followed by proportional changes of the HAB-basic protein content. After the HAB reaction, the Feulgen reaction can be performed on the same slide.

Original languageEnglish
Pages (from-to)203-209
Number of pages7
JournalActa Histochemica
Volume58
Issue number2
Publication statusPublished - 1977

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Alcian Blue
Heparin
Staining and Labeling
Proteins
Micropore Filters
Ehrlich Tumor Carcinoma
Deamination
Histones
Gels
DNA

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cytophotometric determination of basic proteins with heparin alcian blue method. / Pokorny, E.; Szentirmay, Z.; Sugar, J.

In: Acta Histochemica, Vol. 58, No. 2, 1977, p. 203-209.

Research output: Contribution to journalArticle

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